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Vitellogenin levels in hemolymph, ovary and hepatopancreas of the freshwater crayfish Cherax quadricarinatus (Decapoda: Parastacidae) during the reproductive cycle
Ferré, Lilian E.; Medesani, Daniel A.; García, C. Fernando; Grodzielski, Matías; Rodríguez, Enrique M..
  • Ferré, Lilian E.; University of Buenos Aires. Dept. of Biodiversity and Experimental Biology, DBBE-FCEyN. AR
  • Medesani, Daniel A.; University of Buenos Aires. Dept. of Biodiversity and Experimental Biology, DBBE-FCEyN. AR
  • García, C. Fernando; Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP). AR
  • Grodzielski, Matías; University of Buenos Aires. Dept. of Biodiversity and Experimental Biology, DBBE-FCEyN. AR
  • Rodríguez, Enrique M.; University of Buenos Aires. Dept. of Biodiversity and Experimental Biology, DBBE-FCEyN. AR
Rev. biol. trop ; 60(1): 253-261, Mar. 2012. ilus, graf, tab
Article in English | LILACS | ID: lil-657776
Responsible library: CR1.1
ABSTRACT
The freshwater crayfish Cherax quadricarinatus is a tropical species of great interest for aquaculture. Vitellogenin (Vg), a lipoprotein precursor of the vitellum accumulated in spawned eggs, can be synthesized in the ovary and/or hepatopancreas of most crustaceans, being the hemolymph the way for transporting Vg throughout the reproductive cycle. Concentration of Vg in hemolymph, ovary and hepatopancreas of Cherax quadricarinatus adult females was measured by means of ELISA, specifically developed after purifying the native Vg. Measurements were made at four periods of the reproductive cycle pre-reproductive, mid-reproductive, late reproductive and post-reproductive. Besides, both hepatosomatic (HSI) and gonadosomatic (GSI) indexes were determined in each period. Significant variations in Vg levels were detected in both hemolymph and hepatopancreas, being the highest values observed during the mid-reproductive period. Besides, such variations were positively correlated to the HSI. A positive correlation between Vg levels in hepatopancreas and ovary was also seen. These results support previous evidences about the central role of the hepatopancreas as a site of Vg synthesis in the studied species, together with the relevancy of hemolymph for transporting Vg from the hepatopancreas to the ovary. For aquaculture purposes, Vg monitoring in hemolymph could be used as a non-injurious method, to check the reproductive activity of C. quadricarinatus females.
RESUMEN
La langosta de agua dulce Cherax quadricarinatus es una especie tropical de gran interés para la acuicultura. Se midió la concentración de vitelogenina (Vg) en hemolinfa, ovario y hepatopáncreas de hembras adultas de esta especie, por medio de ELISA. Las mediciones fueron hechas en los cuatro períodos del ciclo reproductivo pre-reproductivo, reproductivo medio, reproductivo tardío y post-reproductivo. Se detectaron variaciones significativas en los niveles de Vg tanto en hemolinfa como en hepatopáncreas, se observó el mayor valor durante el período reproductivo medio. Además, tales variaciones se correlacionaron positivamente con el índice hepatosomático. Se observó además una correlación positiva de los niveles de Vg entre hepatopáncreas y ovario. Estos resultados apoyan evidencias previas sobre el papel central del hepatopáncreas como sitio de síntesis de Vg, en esta especie, y también enfatizan la importancia de la hemolinfa para el transporte de la Vg del hepatopáncreas al ovario. Para propósitos de acuicultura, la medición de Vg en hemolinfa podría ser utilizada como un método no lesivo, con el fin de constatar la actividad reproductiva de hembras de C. quadricarinatus.
Subject(s)
Full text: Available Index: LILACS (Americas) Main subject: Ovary / Hemolymph / Vitellogenins / Astacoidea / Hepatopancreas Limits: Animals Language: English Journal: Rev. biol. trop Journal subject: Biology / Tropical Medicine Year: 2012 Type: Article Affiliation country: Argentina Institution/Affiliation country: Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP)/AR / University of Buenos Aires/AR

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Full text: Available Index: LILACS (Americas) Main subject: Ovary / Hemolymph / Vitellogenins / Astacoidea / Hepatopancreas Limits: Animals Language: English Journal: Rev. biol. trop Journal subject: Biology / Tropical Medicine Year: 2012 Type: Article Affiliation country: Argentina Institution/Affiliation country: Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP)/AR / University of Buenos Aires/AR