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Identification of Lactobacillus spp. in colostrum from Chilean mothers
Dubos, Céline; Vega, Natalia; Carvallo, Claudia; Navarrete, Paola; Cerda, Carol; Brunser, Oscar; Gotteland, Martin.
  • Dubos, Céline; University of Chile.
  • Vega, Natalia; University of Chile.
  • Carvallo, Claudia; University of Chile.
  • Navarrete, Paola; University of Chile.
  • Cerda, Carol; University of Chile.
  • Brunser, Oscar; University of Chile.
  • Gotteland, Martin; University of Chile.
Arch. latinoam. nutr ; 61(1): 66-68, Jan. 2011. ilus, graf, mapas, tab
Article in English | LILACS | ID: lil-659100
ABSTRACT
The biodiversity of Lactobacillus spp. in colostrum samples from 116 Chilean mothers was analyzed by PCR and 16S rDNA sequencing. Lactobacilli were isolated in 55.3% of the samples, with concentrations of 3.33 ± 0.55 (log CFU/ml). The predominant species were L. plantarum (64%), L. fermentum (16%) and L. pentosus (9%). 28% of the isolated strains were resistant to gastric pH and bile salts, suggesting that they could be used as probiotics.
RESUMEN
Se analizó la biodiversidad de especies de Lactobacillus en muestras de calostro de 116 madres chilenas mediante PCR y secuenciación del rDNA 16S. Se aislaron lactobacilos en 55,3% de las muestras, con concentraciones de 3,33 ± 0,55 (log UFC / ml). Las especies predominantes fueron L. plantarum (64%), L. fermentum (16%) y L. pentosus (9%). 28% de las cepas aisladas fueron resistentes a pH gástrico y a las sales biliares, lo que sugiere que podrían ser utilizados como probióticos.
Subject(s)

Full text: Available Index: LILACS (Americas) Main subject: Colostrum / Lactobacillus Type of study: Diagnostic study Limits: Female / Humans Country/Region as subject: South America / Chile Language: English Journal: Arch. latinoam. nutr Journal subject: Bioqu¡mica / Educa‡Æo Alimentar e Nutricional / Fen“menos Fisiol¢gicos da Nutri‡Æo / Microbiologia de Alimentos / NUTRICAO Year: 2011 Type: Article Affiliation country: Chile

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Full text: Available Index: LILACS (Americas) Main subject: Colostrum / Lactobacillus Type of study: Diagnostic study Limits: Female / Humans Country/Region as subject: South America / Chile Language: English Journal: Arch. latinoam. nutr Journal subject: Bioqu¡mica / Educa‡Æo Alimentar e Nutricional / Fen“menos Fisiol¢gicos da Nutri‡Æo / Microbiologia de Alimentos / NUTRICAO Year: 2011 Type: Article Affiliation country: Chile