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Overexpression of Fc receptor-like 1 associated with B-cell activation during hepatitis B virus infection
Wang, Ke; Pei, Hao; Huang, Biao; Yang, Run-Lin; Wu, Hang-Yuan; Zhu, Xue; Zhu, Lan.
  • Wang, Ke; Jiangsu Institute of Nuclear Medicine. Jiangsu Key Laboratory of Molecular Nuclear Medicine. Ministry of Health. Key Laboratory of Nuclear Medicine. Wuxi. CN
  • Pei, Hao; Jiangsu Institute of Nuclear Medicine. Jiangsu Key Laboratory of Molecular Nuclear Medicine. Ministry of Health. Key Laboratory of Nuclear Medicine. Wuxi. CN
  • Huang, Biao; Jiangsu Institute of Nuclear Medicine. Jiangsu Key Laboratory of Molecular Nuclear Medicine. Ministry of Health. Key Laboratory of Nuclear Medicine. Wuxi. CN
  • Yang, Run-Lin; Jiangsu Institute of Nuclear Medicine. Jiangsu Key Laboratory of Molecular Nuclear Medicine. Ministry of Health. Key Laboratory of Nuclear Medicine. Wuxi. CN
  • Wu, Hang-Yuan; Jiangsu Institute of Nuclear Medicine. Jiangsu Key Laboratory of Molecular Nuclear Medicine. Ministry of Health. Key Laboratory of Nuclear Medicine. Wuxi. CN
  • Zhu, Xue; Jiangsu Institute of Nuclear Medicine. Jiangsu Key Laboratory of Molecular Nuclear Medicine. Ministry of Health. Key Laboratory of Nuclear Medicine. Wuxi. CN
  • Zhu, Lan; Jiangsu Institute of Nuclear Medicine. Jiangsu Key Laboratory of Molecular Nuclear Medicine. Ministry of Health. Key Laboratory of Nuclear Medicine. Wuxi. CN
Braz. j. med. biol. res ; 45(12): 1112-1118, Dec. 2012. ilus, tab
Article in English | LILACS | ID: lil-659635
ABSTRACT
The role of B cells in the pathogenesis of hepatitis B virus (HBV) infection has not been explored in depth. In the present study, the activation status of B cells from peripheral blood of healthy controls (N = 20) and patients with acute hepatitis B (AHB, N = 15) or chronic hepatitis B (CHB, N = 30) was evaluated by measuring the expression levels of B-cell activation markers CD69 and CD86, using quantitative real-time PCR and flow cytometry. Moreover, the potential mechanism underlying B-cell activation during HBV infection was further investigated by analyzing the expression profile of FCRL1, an intrinsic activation molecule of B cells. An elevation in the levels of B-cell activation markers including CD69 and CD86 was observed in the AHB patients (44.31 ± 9.27, 27.64 ± 9.26%) compared to CHB patients (30.35 ± 11.27, 18.41 ± 6.56%, P < 0.05), which was still higher than healthy controls (12.23 ± 7.84, 8.22 ± 3.43%, P < 0.05). Furthermore, the expression of FCRL1 was found to be similar to B-cell activation markers, which was highest in AHB patients (70.15 ± 17.11%), lowest in healthy donors (36.32 ± 9.98%, P < 0.05) and half-way between these levels in patients with CHB (55.17 ± 12.03%, P < 0.05). The results were positively associated with aberrant B-cell activation. These data suggest that B cells can play a role in HBV infection, and therefore more effort should be devoted to exploring their functions.
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Full text: Available Index: LILACS (Americas) Main subject: B-Lymphocytes / Lymphocyte Activation / Hepatitis B / Membrane Proteins Type of study: Observational study / Risk factors Limits: Adult / Female / Humans / Male Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2012 Type: Article Affiliation country: China Institution/Affiliation country: Jiangsu Institute of Nuclear Medicine/CN

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Full text: Available Index: LILACS (Americas) Main subject: B-Lymphocytes / Lymphocyte Activation / Hepatitis B / Membrane Proteins Type of study: Observational study / Risk factors Limits: Adult / Female / Humans / Male Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2012 Type: Article Affiliation country: China Institution/Affiliation country: Jiangsu Institute of Nuclear Medicine/CN