Presencia y persistencia de variantes moleculares del ORF E6 y ORF E7 del VPH 58 en mujeres con citología normal que pertenecen a la cohorte de Bogotá, Colombia / Presence and persistence of HPV 58 molecular variations ORF E6 and ORF E7 among women with normal cytology in the Bogotá, Colombia cohort
Rev. colomb. cancerol
;
16(1): 40-48, mar. 2012. graf
Article
in Spanish
| LILACS
| ID: lil-662981
RESUMEN
Objetivo:
Analizar la presencia y persistencia de variantes en E6/E7/VPH 58 en muestras de mujeres con infecciones prevalentes por VPH 58, con citología normal, que pertenecen a la cohorte de Bogotá, Colombia.Métodos:
Se utilizaron cepillados cervicales de 34 mujeres VPH 58, con citología normal, pertenecientes a la línea de base de la cohorte, con su respectivo seguimiento. Se amplificó la región E6/E7 del VPH 58 usando los iniciadores E6F1-E7R1 y los iniciadores E7P1-E7P2. Para el análisis de las variantes se utilizó la técnica de secuencia automática directa. La secuencia referencia del VPH 58 se utilizó para comparar las secuencias obtenidas.Resultados:
En 27/34 muestras se lograron detectar variantes de E6/E7 de VPH 58. En total, se detectaron cinco variantes diferentes, dos de ellas nunca antes reportadas (A169/T307/A694/G744/A761 y T307/A694/G744/A761/G763). Los análisis de eliminación mostraron que el 75% de las variantes se habían eliminado antes de los dos años de seguimiento, y todas las variantes ya se habían eliminado a los seis años de seguimiento.Conclusiones:
Dos nuevas variantes se reportaron a escala mundial de gran relevancia en los ámbitos filogenético y epidemiológico.ABSTRACT
Objective:
To analyze the presence and persistence of E6/E7 HPV58 variations in women with prevalent HPV 58 infection, with normal cytology, who belong to the Bogotá, Colombia cohort.Methods:
Cervical cytobrush was used on 34 HPV58 women, with normal cytology, who are part of the cohort base line; respective follow was performed. The HPV58 E67/E7 region was broadened by using E6F1-E7R1 and E7P1-E7P2 indicators. Variation analysis was carried out with automatic direct sequencing. HPV58 sequence reference was used to compare the sequences that had been obtained.Results:
In 27/34 samples, E6/E7 variations of HPV58 were successfully detected. A total of five different variations were detected, two of which had never been reported before (A169/T307/A694/G744/A761 and T307/A694/G744/A761/G763). Elimination analysis revealed that 75% of variations had been eliminated within two years of follow up, and that all variation had been eliminated at the end of six years of follow up.Conclusions:
Two new variations of universal phylogenetic and epidemiologic noteworthiness were reported.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Epidemiologic Studies
/
Uterine Cervical Neoplasms
/
Cervix Uteri
/
Cross-Sectional Studies
/
Cohort Studies
/
Papillomavirus Infections
Type of study:
Etiology study
/
Incidence study
/
Observational study
/
Prevalence study
/
Prognostic study
/
Risk factors
Limits:
Adolescent
/
Adult
/
Aged
/
Female
/
Humans
Country/Region as subject:
South America
/
Colombia
Language:
Spanish
Journal:
Rev. colomb. cancerol
Journal subject:
Neoplasias
/
Oncologia
Year:
2012
Type:
Article
Affiliation country:
Colombia
Institution/Affiliation country:
Instituto Nacional de Cancerología/CO
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