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Zoonotic potential of multidrug-resistant extraintestinal pathogenic Escherichia coli obtained from healthy poultry carcasses in Salvador, Brazil
Lima-Filho, José Vitor; Martins, Liliane Vilela; Nascimento, Danielle Cristina de Oliveira; Ventura, Roberta Ferreira; Batista, Jacqueline Ellen Camelo; Silva, Ayrles Fernanda Brandão; Ralph, Maria Taciana; Vaz, Renata Valença; Rabello, Carlos Boa-Viagem; Silva, Isabella de Matos Mendes da; Evêncio-Neto, Joaquim.
  • Lima-Filho, José Vitor; Universidade Federal Rural de Pernambuco. Department of Biology. Recife. BR
  • Martins, Liliane Vilela; Universidade Federal Rural de Pernambuco. Department of Biology. Recife. BR
  • Nascimento, Danielle Cristina de Oliveira; Universidade Federal Rural de Pernambuco. Department of Biology. Recife. BR
  • Ventura, Roberta Ferreira; Universidade Federal Rural de Pernambuco. Department of Biology. Recife. BR
  • Batista, Jacqueline Ellen Camelo; Universidade Federal Rural de Pernambuco. Department of Biology. Recife. BR
  • Silva, Ayrles Fernanda Brandão; Universidade Federal Rural de Pernambuco. Department of Biology. Recife. BR
  • Ralph, Maria Taciana; Universidade Federal Rural de Pernambuco. Department of Biology. Recife. BR
  • Vaz, Renata Valença; Universidade Federal Rural de Pernambuco. Department of Biology. Recife. BR
  • Rabello, Carlos Boa-Viagem; Universidade Federal Rural de Pernambuco. Department of Biology. Recife. BR
  • Silva, Isabella de Matos Mendes da; Universidade Federal Rural de Pernambuco. Department of Biology. Recife. BR
  • Evêncio-Neto, Joaquim; Universidade Federal Rural de Pernambuco. Department of Biology. Recife. BR
Braz. j. infect. dis ; 17(1): 54-61, Jan.-Feb. 2013. ilus, tab
Article in English | LILACS | ID: lil-665775
ABSTRACT
The zoonotic potential to cause human and/or animal infections among multidrug-resistant extraintestinal pathogenic Escherichia coli from avian origin was investigated. Twenty-seven extraintestinal pathogenic E. coli isolates containing the increased survival gene (iss) were obtained from the livers of healthy and diseased poultry carcasses at two slaughterhouses in Salvador, northeastern Brazil. The antimicrobial resistance-susceptibility profiles were conducted with antibiotics of avian and/or human use by the standardized disc-diffusion method. Antimicrobial resistance was higher for levofloxacin (51.8%), amoxicillin/clavulanic acid (70.4%), ampicillin (81.5%), cefalotin (88.8%), tetracycline (100%) and streptomycin (100%). The minimum inhibitory concentrations above the resistance breakpoints of doxycycline, neomycin, oxytetracycline and enrofloxacin reached, respectively, 88.0%, 100%, 75% and 91.7% of the isolates. Strains with high and low antimicrobial resistance were i.p. administered to Swiss mice, and histopathological examination was carried out seven days after infection. Resistance to goat and human serum complement was also evaluated. The results show that Swiss mice challenged with strain 2B (resistant to 11 antimicrobials) provoked a severe degeneration of hepatocytes besides lymphocytic infiltration in the liver, whereas the spleen showed areas of degeneration of the white and red pulp. Conversely, the spleen and liver of mice challenged with strain 4A (resistant to two antimicrobials) were morphologically preserved. In addition, complement resistance to goat and human serum was high for strain 2B and low for strain 4A. Our data show that multidrug resistance and pathogenesis can be correlated in extraintestinal pathogenic E. coli strains obtained from apparently healthy poultry carcasses, increasing the risk for human public healthy.
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Full text: Available Index: LILACS (Americas) Main subject: Poultry / Zoonoses / Drug Resistance, Multiple, Bacterial / Escherichia coli / Escherichia coli Infections / Anti-Bacterial Agents Type of study: Prognostic study Limits: Animals Country/Region as subject: South America / Brazil Language: English Journal: Braz. j. infect. dis Journal subject: Communicable Diseases Year: 2013 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal Rural de Pernambuco/BR

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Full text: Available Index: LILACS (Americas) Main subject: Poultry / Zoonoses / Drug Resistance, Multiple, Bacterial / Escherichia coli / Escherichia coli Infections / Anti-Bacterial Agents Type of study: Prognostic study Limits: Animals Country/Region as subject: South America / Brazil Language: English Journal: Braz. j. infect. dis Journal subject: Communicable Diseases Year: 2013 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal Rural de Pernambuco/BR