Behavior of the P1.HTR mastocytoma cell line implanted in the chorioallantoic membrane of chick embryos
Braz. j. med. biol. res
;
46(1): 52-57, 11/jan. 2013. graf
Article
in English
| LILACS
| ID: lil-665791
ABSTRACT
The P1.HTR cell line includes highly transfectable cells derived from P815 mastocytoma cells originating from mouse breast tissue. Despite its widespread use in immunogenic studies, no data are available about the behavior of P1.HTR cells in the chick embryo chorioallantoic membrane model. The objective of the present investigation was to study the effects of P1.HTR cells implanted on the chorioallantoic membrane of chick embryos. We inoculated P1.HTR cells into the previously prepared chick embryo chorioallantoic membrane and observed the early and late effects of these cells by stereomicroscopy, histochemistry and immunohistochemistry. A highly angiotropic and angiogenic effect occurred early after inoculation and a tumorigenic potential with the development of mastocytoma keeping well mast cells immunophenotype was detected later during the development. The P1.HTR mastocytoma cell line is a good tool for the development of the chick embryo chorioallantoic membrane mastocytoma model and also for other studies concerning the involvement of blood vessels. The chick embryo chorioallantoic membrane model of mastocytoma retains the mast cell immunophenotype under experimental conditions and could be used as an experimental tool for in vivo preliminary testing of antitumor and antivascular drugs.
Full text:
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Index:
LILACS (Americas)
Main subject:
Mastocytoma
/
Chorioallantoic Membrane
Limits:
Animals
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
2013
Type:
Article
/
Project document
Affiliation country:
Romania
Institution/Affiliation country:
Victor BabeÅY University of Medicine and Pharmacy/RO
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