Mycoflora study in a wheat flour mill of Argentina
Braz. j. microbiol
;
43(4): 1444-1451, Oct.-Dec. 2012. tab
Article
in English
| LILACS
| ID: lil-665831
ABSTRACT
The mycoflora of the environment wheat conditioning, milling and screening, and filling zone, as well as, raw material -wheat-, intermediate product -grits- and end product -flour- on day 1, and after cleaning improvements -days 45 and 90- were studied in an Argentine wheat mill. Samples were incubated at 28°C for 5-7 days on Malt Extract Agar with chloramphenicol (100 mg L-1) and the results were expressed in colony forming units per cubic meter of air (CFU m-3) or per gram of sample (CFU g-1), respectively. Fungal genera and species were isolated and identified and the potential toxicogenic capacity of the Aspergillus flavus and Fusarium graminearum isolated was studied. Time-Place and Time-Product multifactorial ANOVA were carried out. After cleaning improvements, CFU m-3 of air decreased as a function of time. Cladosporium and Alternaria were abundant in every zone, Aspergillus predominated in the wheat conditioning zone and Penicillium and Eurotium decreased with time. Wheat was more contaminated than grits and flour; Aspergillus, Eurotium and Mucoraceae family were the most abundant. Deoxynivalenol was above the levels allowed in wheat, being acceptable in grits and flour. Aflatoxin and Zearalenone showed acceptable levels. When studied in vitro, 53% of Aspergillus flavus and 100% of Fusarium graminearum isolates, produced Total Aflatoxins, and Deoxynivalenol and Zearalenone, respectively.
Full text:
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Index:
LILACS (Americas)
Main subject:
In Vitro Techniques
/
Flora
/
Aflatoxins
/
Mitosporic Fungi
/
Flour
/
Food Analysis
Country/Region as subject:
South America
/
Argentina
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2012
Type:
Article
Affiliation country:
Argentina
Institution/Affiliation country:
Universidad Nacional del Litoral/AR
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