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Evaluation of histological changes after tracheal occlusion at different gestational ages in a fetal rat model
Gallindo, Rodrigo Melo; Gonçalves, Frances Lilian Lanhellas; Barreto, Carolina Teixeira de Resende; Schmidt, Augusto Frederico Santos; Pereira, Luis Antonio Violin Dias; Sbragia, Lourenço.
  • Gallindo, Rodrigo Melo; University of São Paulo. School of Medicine of Ribeirão Preto. Division of Pediatric Surgery. Department of Surgery and Anatomy. Ribeirão Preto. BR
  • Gonçalves, Frances Lilian Lanhellas; University of São Paulo. School of Medicine of Ribeirão Preto. Division of Pediatric Surgery. Department of Surgery and Anatomy. Ribeirão Preto. BR
  • Barreto, Carolina Teixeira de Resende; University of São Paulo. School of Medicine of Ribeirão Preto. Division of Pediatric Surgery. Department of Surgery and Anatomy. Ribeirão Preto. BR
  • Schmidt, Augusto Frederico Santos; University of São Paulo. School of Medicine of Ribeirão Preto. Division of Pediatric Surgery. Department of Surgery and Anatomy. Ribeirão Preto. BR
  • Pereira, Luis Antonio Violin Dias; University of São Paulo. School of Medicine of Ribeirão Preto. Division of Pediatric Surgery. Department of Surgery and Anatomy. Ribeirão Preto. BR
  • Sbragia, Lourenço; University of São Paulo. School of Medicine of Ribeirão Preto. Division of Pediatric Surgery. Department of Surgery and Anatomy. Ribeirão Preto. BR
Clinics ; 68(1): 59-63, Jan. 2013. ilus, graf, tab
Article in English | LILACS | ID: lil-665918
ABSTRACT

OBJECTIVES:

To evaluate the histological changes of tracheal cartilage and epithelium caused by tracheal occlusion at different gestational ages in a fetal rat model.

METHODS:

Rat fetuses were divided into two groups a) External control, composed of non-operated rats, and b) Interventional group, composed of rats operated upon on gestational day 18.5 (term = 22 days), divided into triads 1) Tracheal occlusion, 2) Internal control and 3) Sham (manipulated but not operated). Morphological data for body weight, total lung weight and total lung weight/body weight ratio were collected and measured on gestational days 19.5, 20.5 and 21.5. Tracheal samples were histologically processed, and epithelial, chondral and total tracheal thicknesses were measured on each gestational day.

RESULTS:

The tracheal occlusion group exhibited an increase in total lung weight/body weight ratio (p<0.001). Histologically, this group had a thicker epithelial thickness (p<0.05) and thinner chondral (p<0.05) and total tracheal thicknesses (p<0.001). These differences were more prominent on gestational days 20.5 and 21.5.

CONCLUSION:

Tracheal occlusion changed tracheal morphology, increased epithelial thickness and considerably decreased total tracheal thickness. These changes in the tracheal wall could explain the development of tracheomegaly, recently reported in some human fetuses subjected to tracheal occlusion.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Trachea / Gestational Age / Models, Animal / Fetus / Therapeutic Occlusion Type of study: Evaluation studies / Prognostic study Limits: Animals Language: English Journal: Clinics Journal subject: Medicine Year: 2013 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: University of São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Trachea / Gestational Age / Models, Animal / Fetus / Therapeutic Occlusion Type of study: Evaluation studies / Prognostic study Limits: Animals Language: English Journal: Clinics Journal subject: Medicine Year: 2013 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: University of São Paulo/BR