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Regulatory volume decrease in Leishmania mexicana: effect of anti-microtubule drugs
Dagger, Francehuli; Valdivieso, Elizabeth; Marcano, Ana K; Ayesta, Carlos.
  • Dagger, Francehuli; Universidad Central de Venezuela. Facultad de Ciencias. Instituto de Biología Experimental. Laboratorio de Biología Celular de Parásitos. Caracas. VE
  • Valdivieso, Elizabeth; Universidad Central de Venezuela. Facultad de Ciencias. Instituto de Biología Experimental. Laboratorio de Biología Celular de Parásitos. Caracas. VE
  • Marcano, Ana K; Universidad Central de Venezuela. Facultad de Ciencias. Instituto de Biología Experimental. Laboratorio de Biología Celular de Parásitos. Caracas. VE
  • Ayesta, Carlos; Universidad Central de Venezuela. Facultad de Ciencias. Instituto de Biología Experimental. Laboratorio de Biología Celular de Parásitos. Caracas. VE
Mem. Inst. Oswaldo Cruz ; 108(1): 84-90, Feb. 2013. ilus, graf, tab
Article in English | LILACS | ID: lil-666049
ABSTRACT
The trypanosomatid cytoskeleton is responsible for the parasite's shape and it is modulated throughout the different stages of the parasite's life cycle. When parasites are exposed to media with reduced osmolarity, they initially swell, but subsequently undergo compensatory shrinking referred to as regulatory volume decrease (RVD). We studied the effects of anti-microtubule (Mt) drugs on the proliferation of Leishmania mexicana promastigotes and their capacity to undergo RVD. All of the drugs tested exerted antiproliferative effects of varying magnitudes [ansamitocin P3 (AP3)> trifluoperazine > taxol > rhizoxin > chlorpromazine]. No direct relationship was found between antiproliferative drug treatment and RVD. Similarly, Mt stability was not affected by drug treatment. Ansamitocin P3, which is effective at nanomolar concentrations, blocked amastigote-promastigote differentiation and was the only drug that impeded RVD, as measured by light dispersion. AP3 induced 2 kinetoplasts (Kt) 1 nucleus cells that had numerous flagella-associated Kts throughout the cell. These results suggest that the dramatic morphological changes induced by AP3 alter the spatial organisation and directionality of the Mts that are necessary for the parasite's hypotonic stress-induced shape change, as well as its recovery.
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Full text: Available Index: LILACS (Americas) Main subject: Cytoskeleton / Leishmania mexicana / Tubulin Modulators Limits: Animals Country/Region as subject: Mexico Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2013 Type: Article / Project document Affiliation country: Venezuela Institution/Affiliation country: Universidad Central de Venezuela/VE

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Full text: Available Index: LILACS (Americas) Main subject: Cytoskeleton / Leishmania mexicana / Tubulin Modulators Limits: Animals Country/Region as subject: Mexico Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2013 Type: Article / Project document Affiliation country: Venezuela Institution/Affiliation country: Universidad Central de Venezuela/VE