Production and diagnostic application of recombinant domain III of West Nile envelope protein in Brazil
Rev. Soc. Bras. Med. Trop
;
46(1): 97-99, Jan.-Feb. 2013. ilus, tab
Article
in English
| LILACS
| ID: lil-666813
ABSTRACT
INTRODUCTION:
West Nile virus (WNV) is a flavivirus with a natural cycle involving mosquitoes and birds. Over the last 11 years, WNV has spread throughout the Americas with the imminent risk of its introduction in Brazil.METHODS:
Envelope protein domain III of WNV (rDIII) was bacterially expressed and purified. An enzyme-linked immunosorbent assay with WNV rDIII antigen was standardized against mouse immune fluids (MIAFs) of different flavivirus.RESULTS:
WNV rDIII reacted strongly with St. Louis encephalitis virus (SLEV) MIAF but not with other flaviviruses.CONCLUSIONS:
This antigen may be a potentially useful tool for serologic diagnosis and may contribute in future epidemiological surveillance of WNV infections in Brazil.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
West Nile virus
/
Recombinant Proteins
/
Viral Envelope Proteins
/
Antibodies, Neutralizing
/
Antibodies, Viral
Type of study:
Diagnostic study
Limits:
Animals
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Rev. Soc. Bras. Med. Trop
Journal subject:
Tropical Medicine
Year:
2013
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade de São Paulo/BR
Similar
MEDLINE
...
LILACS
LIS