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Production and diagnostic application of recombinant domain III of West Nile envelope protein in Brazil
Chávez, Juliana Helena; Reis, Vinicius Pinho dos; Silva, Jaqueline Raymondi; Laure, Helen Julie; Rosa, José Cesar; Fonseca, Benedito Antônio Lopes da; Figueiredo, Luiz Tadeu Moraes.
  • Chávez, Juliana Helena; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Centro de Pesquisa em Virologia. Ribeirão Preto. BR
  • Reis, Vinicius Pinho dos; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Centro de Pesquisa em Virologia. Ribeirão Preto. BR
  • Silva, Jaqueline Raymondi; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Centro de Pesquisa em Virologia. Ribeirão Preto. BR
  • Laure, Helen Julie; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Centro de Pesquisa em Virologia. Ribeirão Preto. BR
  • Rosa, José Cesar; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Centro de Pesquisa em Virologia. Ribeirão Preto. BR
  • Fonseca, Benedito Antônio Lopes da; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Centro de Pesquisa em Virologia. Ribeirão Preto. BR
  • Figueiredo, Luiz Tadeu Moraes; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Centro de Pesquisa em Virologia. Ribeirão Preto. BR
Rev. Soc. Bras. Med. Trop ; 46(1): 97-99, Jan.-Feb. 2013. ilus, tab
Article in English | LILACS | ID: lil-666813
ABSTRACT

INTRODUCTION:

West Nile virus (WNV) is a flavivirus with a natural cycle involving mosquitoes and birds. Over the last 11 years, WNV has spread throughout the Americas with the imminent risk of its introduction in Brazil.

METHODS:

Envelope protein domain III of WNV (rDIII) was bacterially expressed and purified. An enzyme-linked immunosorbent assay with WNV rDIII antigen was standardized against mouse immune fluids (MIAFs) of different flavivirus.

RESULTS:

WNV rDIII reacted strongly with St. Louis encephalitis virus (SLEV) MIAF but not with other flaviviruses.

CONCLUSIONS:

This antigen may be a potentially useful tool for serologic diagnosis and may contribute in future epidemiological surveillance of WNV infections in Brazil.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: West Nile virus / Recombinant Proteins / Viral Envelope Proteins / Antibodies, Neutralizing / Antibodies, Viral Type of study: Diagnostic study Limits: Animals Country/Region as subject: South America / Brazil Language: English Journal: Rev. Soc. Bras. Med. Trop Journal subject: Tropical Medicine Year: 2013 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: West Nile virus / Recombinant Proteins / Viral Envelope Proteins / Antibodies, Neutralizing / Antibodies, Viral Type of study: Diagnostic study Limits: Animals Country/Region as subject: South America / Brazil Language: English Journal: Rev. Soc. Bras. Med. Trop Journal subject: Tropical Medicine Year: 2013 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade de São Paulo/BR