Effects of silibinin and ethanol on skeletal muscle ischemia-reperfusion injury
Acta cir. bras
;
28(3): 179-184, Mar. 2013. ilus
Article
in English
| LILACS
| ID: lil-667927
ABSTRACT
PURPOSE:
To investigate the potential beneficial effect of silibinin in ischemia-reperfusion injury (IRI) of skeletal muscle.METHODS:
Under urethane anesthesia, four experimental groups were established in Balb/c mice I) Sham-control, II) IRI (Tourniquet-induced) (2+1 h), III) IRI+ethanol (10%), and IV) IRI+silibinin (50 mg/kg/IP). The viability of muscle (left) was evaluated by the triphenyltetrazolium chloride dye method and calculated as the percentage of the contralateral control muscle (right). Malondialdehyde, superoxide dismutase, and catalase were measured in the gastrocnemius muscle via a spectrophotometer.RESULTS:
The viability of gastrocnemius muscle in group II was significantly lower in comparison with that seen in group I. The administration of either ethanol or silibinin rendered the tissues to recover nearly to the baseline level. Additionally, malondialdehyde levels were higher in group II than those in group I. The application of silibinin prior to the reperfusion attenuated these to the control levels. However, malondialdehyde levels in the ethanol administrated group were reduced as well. The enhanced superoxide dismutase activity seen in the IRI group was not diminished in the animals treated with either silibinin or ethanol. Similarly, there were no differences between groups regarding the catalase activities.CONCLUSION:
Ethanol seems to be effective in attenuating IRI in skeletal muscle and no definite conclusion can be made on silibinin effect.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Silymarin
/
Reperfusion Injury
/
Muscle, Skeletal
/
Ethanol
/
Antioxidants
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2013
Type:
Article
Affiliation country:
Turkey
Institution/Affiliation country:
Kahramanmaraş Sütçü İmam University/TR
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