Evaluating Colombian Clostridium spp. Strains hydrogen production using glycerol as substrate
Electron. j. biotechnol
;
16(2): 5-5, Mar. 2013. ilus, tab
Article
in English
| LILACS
| ID: lil-670133
ABSTRACT
Background:
The ability for hydrogen production of 13 native strains of Clostridium spp. strains isolated from Colombian soil was evaluated using glycerol substrate. Glycerol to hydrogen conversion was investigated using a batch fermentation reactor and industrial glycerol source (50 g.l-1, pH 7.00).Results:
The results were quantified regarding acids, hydrogen, biomass and solvent production. The selected strain gave good hydrogen over production output at 14.4 mmol H2.l-1, productivity 0.3167 mg H2.h-1 l-1 culture mediumand yield 0.1962 mol H2.mol-1 glycerol. A further fermentation assay a 4.0 liter batch reactor let to being 0.26 mg.l-1.h-1 after 18 hrs of fermentation. Logistic model, Luedeking-Piret model and Luedeking-Piret modified models were used for modeling changes in cell growth, hydrogen production and substrate consumption (Correlation coefficients R² = 0.95 for biomass substrate, R² = 0.77 hydrogen production).Conclusions:
Our results indicate that hydrogen production through glycerol bioconversion by native strains is possible.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Clostridium
/
Hydrogen
Country/Region as subject:
South America
/
Colombia
Language:
English
Journal:
Electron. j. biotechnol
Journal subject:
Biotechnology
Year:
2013
Type:
Article
Affiliation country:
Colombia
Institution/Affiliation country:
Universidad Nacional/CO
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