Your browser doesn't support javascript.
loading
A human astrocytoma cell line is highly susceptible to infection with Trypanosoma cruzi
Memórias do Instituto Oswaldo Cruz; Vargas-Zambrano, Juan Camilo; Lasso, Paola; Cuellar, Adriana; Puerta, Concepción Judith; González, John Mario.
  • Vargas-Zambrano, Juan Camilo; Universidad de los Andes. Facultad de Medicina. Grupo de Ciencias Básicas Médicas. Bogotá. CO
  • Lasso, Paola; Universidad de los Andes. Facultad de Medicina. Grupo de Ciencias Básicas Médicas. Bogotá. CO
  • Cuellar, Adriana; Universidad de los Andes. Facultad de Medicina. Grupo de Ciencias Básicas Médicas. Bogotá. CO
  • Puerta, Concepción Judith; Universidad de los Andes. Facultad de Medicina. Grupo de Ciencias Básicas Médicas. Bogotá. CO
  • González, John Mario; Universidad de los Andes. Facultad de Medicina. Grupo de Ciencias Básicas Médicas. Bogotá. CO
Mem. Inst. Oswaldo Cruz ; 108(2): 212-219, abr. 2013. graf
Article in English | LILACS | ID: lil-670398
ABSTRACT
Astrocytes play a vital role in neuronal protection, homeostasis, vascular interchange and the local immune response. Some viruses and parasites can cross the blood-brain barrier and infect glia. Trypanosoma cruzi, the aetiological agent of Chagas disease, can seriously compromise the central nervous system, mainly in immune-suppressed individuals, but also during the acute phase of the infection. In this report, the infective capacity of T. cruzi in a human astrocyte tumour-derived cell line was studied. Astrocytes exposed to trypomastigotes (110 ratio) produced intracellular amastigotes and new trypomastigotes emerged by day 4 post-infection (p.i.). At day 6 p.i., 93% of the cells were infected. Using flow cytometry, changes were observed in both the expression of major histocompatibility complex class I and II molecules and the chemokine secretion pattern of astrocytes exposed to the parasite. Blocking the low-density lipoprotein receptor on astrocytes did not reduce parasite intracellular infection. Thus, T. cruzi can infect astrocytes and modulate the immune response during central nervous system infection.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Astrocytoma / Trypanosoma cruzi / Astrocytes / Immunity, Cellular Limits: Humans Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2013 Type: Article Affiliation country: Colombia Institution/Affiliation country: Universidad de los Andes/CO

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Index: LILACS (Americas) Main subject: Astrocytoma / Trypanosoma cruzi / Astrocytes / Immunity, Cellular Limits: Humans Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2013 Type: Article Affiliation country: Colombia Institution/Affiliation country: Universidad de los Andes/CO