A human astrocytoma cell line is highly susceptible to infection with Trypanosoma cruzi
Mem. Inst. Oswaldo Cruz
;
108(2): 212-219, abr. 2013. graf
Article
in English
| LILACS
| ID: lil-670398
ABSTRACT
Astrocytes play a vital role in neuronal protection, homeostasis, vascular interchange and the local immune response. Some viruses and parasites can cross the blood-brain barrier and infect glia. Trypanosoma cruzi, the aetiological agent of Chagas disease, can seriously compromise the central nervous system, mainly in immune-suppressed individuals, but also during the acute phase of the infection. In this report, the infective capacity of T. cruzi in a human astrocyte tumour-derived cell line was studied. Astrocytes exposed to trypomastigotes (110 ratio) produced intracellular amastigotes and new trypomastigotes emerged by day 4 post-infection (p.i.). At day 6 p.i., 93% of the cells were infected. Using flow cytometry, changes were observed in both the expression of major histocompatibility complex class I and II molecules and the chemokine secretion pattern of astrocytes exposed to the parasite. Blocking the low-density lipoprotein receptor on astrocytes did not reduce parasite intracellular infection. Thus, T. cruzi can infect astrocytes and modulate the immune response during central nervous system infection.
Full text:
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Index:
LILACS (Americas)
Main subject:
Astrocytoma
/
Trypanosoma cruzi
/
Astrocytes
/
Immunity, Cellular
Limits:
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2013
Type:
Article
Affiliation country:
Colombia
Institution/Affiliation country:
Universidad de los Andes/CO
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