Your browser doesn't support javascript.
loading
Nitric oxide production by Peromyscus yucatanicus (Rodentia) infected with Leishmania (Leishmania) mexicana
Memórias do Instituto Oswaldo Cruz; Loría-Cervera, Elsy Nalleli; Sosa-Bibiano, Erika Ivett; Villanueva-Lizama, Liliana Estefanía; Van Wynsberghe, Nicole Raymonde; Canto-Lara, Silvia Beatriz; Batún-Cutz, José Luis; Andrade-Narváez, Fernando José.
  • Loría-Cervera, Elsy Nalleli; Universidad Autónoma de Yucatán. Centro de Investigaciones Regionales Dr Hideyo Noguchi. Laboratorio de Inmunología. Yucatán. MX
  • Sosa-Bibiano, Erika Ivett; Universidad Autónoma de Yucatán. Centro de Investigaciones Regionales Dr Hideyo Noguchi. Laboratorio de Inmunología. Yucatán. MX
  • Villanueva-Lizama, Liliana Estefanía; Universidad Autónoma de Yucatán. Centro de Investigaciones Regionales Dr Hideyo Noguchi. Laboratorio de Inmunología. Yucatán. MX
  • Van Wynsberghe, Nicole Raymonde; Universidad Autónoma de Yucatán. Centro de Investigaciones Regionales Dr Hideyo Noguchi. Laboratorio de Inmunología. Yucatán. MX
  • Canto-Lara, Silvia Beatriz; Universidad Autónoma de Yucatán. Centro de Investigaciones Regionales Dr Hideyo Noguchi. Laboratorio de Inmunología. Yucatán. MX
  • Batún-Cutz, José Luis; Universidad Autónoma de Yucatán. Centro de Investigaciones Regionales Dr Hideyo Noguchi. Laboratorio de Inmunología. Yucatán. MX
  • Andrade-Narváez, Fernando José; Universidad Autónoma de Yucatán. Centro de Investigaciones Regionales Dr Hideyo Noguchi. Laboratorio de Inmunología. Yucatán. MX
Mem. Inst. Oswaldo Cruz ; 108(2): 172-177, abr. 2013. tab, graf
Article in English | LILACS | ID: lil-670406
ABSTRACT
Peromyscus yucatanicus (Rodentia Cricetidae) is a primary reservoir of Leishmania (Leishmania) mexicana (Kinetoplastida Trypanosomatidae). Nitric oxide (NO) generally plays a crucial role in the containment and elimination of Leishmania. The aim of this study was to determine the amount of NO produced by P. yucatanicus infected with L. (L.) mexicana. Subclinical and clinical infections were established in P. yucatanicus through inoculation with 1 x 10 2 and 2.5 x 10 6 promastigotes, respectively. Peritoneal macrophages were cultured alone or co-cultured with lymphocytes with or without soluble Leishmania antigen. The level of NO production was determined using the Griess reaction. The amount of NO produced was significantly higher (p ≤ 0.0001) in co-cultured macrophages and lymphocytes than in macrophages cultured alone. No differences in NO production were found between P. yucatanicus with subclinical L. (L.) mexicana infections and animals with clinical infections. These results support the hypothesis that the immunological mechanisms of NO production in P. yucatanicus are similar to those described in mouse models of leishmaniasis and, despite NO production, P. yucatanicus is unable to clear the parasite infection.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Leishmania mexicana / Peromyscus / Leishmaniasis, Cutaneous / Macrophages, Peritoneal / Nitric Oxide Limits: Animals Country/Region as subject: Mexico Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2013 Type: Article / Project document Affiliation country: Mexico Institution/Affiliation country: Universidad Autónoma de Yucatán/MX

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Index: LILACS (Americas) Main subject: Leishmania mexicana / Peromyscus / Leishmaniasis, Cutaneous / Macrophages, Peritoneal / Nitric Oxide Limits: Animals Country/Region as subject: Mexico Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2013 Type: Article / Project document Affiliation country: Mexico Institution/Affiliation country: Universidad Autónoma de Yucatán/MX