Your browser doesn't support javascript.
loading
The effects of ingested aluminium on brain cytochrome oxidase activity / Efectos de la ingestión de aluminio sobre la actividad de la oxidasa citocrómica del cerebro
Mohan, N; Alleyne, T; Adogwa, A.
  • Mohan, N; The University of the West Indies. Faculty of Medical Sciences. Department of Pre-Clinical Sciences. Biochemistry Unit. St Augustine. TT
  • Alleyne, T; The University of the West Indies. Faculty of Medical Sciences. Department of Pre-Clinical Sciences. Biochemistry Unit. St Augustine. TT
  • Adogwa, A; The University of the West Indies. Faculty of Medical Sciences. Department of Pre-Clinical Sciences. Biochemistry Unit. St Augustine. TT
West Indian med. j ; 58(5): 422-427, Nov. 2009. ilus, tab
Article in English | LILACS | ID: lil-672514
ABSTRACT
Aluminium has a unique combination of physical and chemical properties which has enabled man to put this metal to very wide and varied use. However, prolonged exposure to aluminium ions may lead to adverse health effects. In this study, we evaluated the effects of dietary aluminium on the protein composition and the intrinsic activity of cytochrome oxidase (COX) for brain mitochondria. New Zealand white rabbits were maintained on a diet of commercial rabbit pellets and distilled water for a period of 12 weeks. For the experimental group, AlCl3, 330mg/kg/L was added to the drinking water. When compared to the control, mitochondria isolated from the brains of the AlCl3 fed rabbits showed no change in Km but an approximate 35% decrease in both the low and high affinity Vmax values. Also, whereas the protein composition of the mitochondria from both sources appeared to be normal, isolation of highly purified COX proved to be difficult and for the AICI3 fed rabbits, a number of the enzyme's low molecular weight subunits were absent. These results appear to confirm a relationship between long term aluminium consumption and low brain COX activity; they further suggest that an altered COX structure may be the cause of the low enzymic activity.
RESUMEN
El aluminio posee una combinación única de las propiedades físicas y químicas que ha permitido al ser humano hacer un uso amplio y variado de este metal. Sin embargo, un número de estudios recientes, sugiere que la exposición prolongada a los iones de aluminio puede tener efectos nocivos sobre la salud. En el presente estudio, evaluamos los efectos del aluminio dietético sobre la composición proteínica y la actividad intrínseca de la oxidasa citocrómica (COX) para la mitocondria cerebral. Conejos blancos de Nueva Zelanda, fueron mantenidos con una dieta de alimento para conejos y agua destilada por un período de 12 semanas. Para el grupo experimental AlCl3, 330mg/kg/L fueron añadidos al agua potable. En comparación con el grupo de control, las mitocondrias aisladas de los cerebros de los conejos alimentados con AlCl3 no mostraron cambios en Km pero hubo una disminución de aproximadamente 35% tanto en los valores Vmax de baja y alta afinidad. Por otro lado, mientras que la composición proteica de las mitocondrias de ambas fuentes parecía ser normal, resultó difícil aislar el COX altamente purificado y un número de enzimas de subunidades de bajo peso molecular MMMM estuvieron ausentes. Estos resultados parecen confirmar una relación entre el consumo de aluminio a largo plazo y la baja actividad del COX del cerebro. Asimismo, sugieren que una alteración de la estructura del COX puede ser la causa de una baja actividad enzimática.
Subject(s)
Full text: Available Index: LILACS (Americas) Main subject: Brain / Chlorides / Aluminum Compounds / Electron Transport Complex IV / Mitochondria Limits: Animals Language: English Journal: West Indian med. j Journal subject: Medicine Year: 2009 Type: Article Affiliation country: Trinidad and Tobago Institution/Affiliation country: The University of the West Indies/TT

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: LILACS (Americas) Main subject: Brain / Chlorides / Aluminum Compounds / Electron Transport Complex IV / Mitochondria Limits: Animals Language: English Journal: West Indian med. j Journal subject: Medicine Year: 2009 Type: Article Affiliation country: Trinidad and Tobago Institution/Affiliation country: The University of the West Indies/TT