Experimental diabetes exacerbates skin transplant rejection in rats
Acta cir. bras
;
28(5): 323-326, May 2013. ilus, tab
Article
in English
| LILACS
| ID: lil-674150
ABSTRACT
PURPOSE:
To investigate the effect of chronic experimental diabetes on skin allografts in rats as a simple model that could clarify some basic aspects and mechanisms involved in transplant rejection in diabetes compared to normal animals.METHODS:
Skin grafting was performed with fragments of tail skin from sex matched non diabetic Wistar rats engrafted onto the thoracic area of diabetic and non diabetic recipients. Grafts were scored for rejection every other day and were removed on day 14. Skin grafts were graded according to the following itens no rejection; or rejection including acute, chronic and humoral and/or cellular rejection. Statistical analysis was performed using JMP 5.1 software with ANOVA test. Diabetes was induced with IV injection of alloxan 40 mg/kg.RESULTS:
Inflammatory vascular infiltrate compromising the endothelium with areas of fibrinoid necrosis and thrombosis characteristics of acute humoral rejection and subendothelial lymphocyte infiltrate typical of acute cellular rejection were significantly (p<0.003) higher in diabetic than in non diabetic recipients as the inflammatory infiltrate in the epidermis (p<0.002).CONCLUSION:
Skin transplant acute rejection from chronic alloxan diabetic rats to normal tissue was significantly more intense than the acute rejection between normal rats.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Skin Transplantation
/
Diabetes Mellitus, Experimental
/
Graft Rejection
Type of study:
Etiology study
/
Prognostic study
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2013
Type:
Article
Affiliation country:
Brazil
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