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Nitric oxide levels in cochlear fluids of guinea pig
Yonamine, Fernando Kaoru; Onishi, Ektor Tsuneo; Mouro, Margareth Gori; Higa, Elisa Mieko Suemitsu; Cruz, Oswaldo Laércio Mendonça.
  • Yonamine, Fernando Kaoru; UNIFESP. Department of Otorhinolaryngology, Head and Neck Surgery.
  • Onishi, Ektor Tsuneo; UNIFESP. Department of Otorhinolaryngology, Head and Neck Surgery.
  • Mouro, Margareth Gori; UNIFESP. Department of Otorhinolaryngology, Head and Neck Surgery.
  • Higa, Elisa Mieko Suemitsu; UNIFESP. Department of Otorhinolaryngology, Head and Neck Surgery.
  • Cruz, Oswaldo Laércio Mendonça; UNIFESP. Department of Otorhinolaryngology, Head and Neck Surgery.
Acta cir. bras ; 28(5): 397-402, May 2013. ilus, tab
Article in English | LILACS | ID: lil-674162
ABSTRACT

PURPOSE:

To describe a technique to obtain guinea pigs cochlear fluids and measure nitric oxide (NO) concentration.

METHODS:

Six guinea pigs were used and sacrificed. The cochlear fluids collected for measurement of NO, performed by chemiluminescence (NOA 280).

RESULTS:

Through the chemiluminescence was possible to analyze the concentration of NO in cochlear fluids obtained. Average levels of nitric oxide from guinea pigs was 12.55 µM.

CONCLUSION:

It is possible to obtain nitric oxide cochlear fluids, with this technique and nitric oxide concentration measure by chemiluminescence, a quantitative and more precise method.
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Full text: Available Index: LILACS (Americas) Main subject: Cochlea / Nitric Oxide Type of study: Prognostic study Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2013 Type: Article Affiliation country: Brazil

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Full text: Available Index: LILACS (Americas) Main subject: Cochlea / Nitric Oxide Type of study: Prognostic study Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2013 Type: Article Affiliation country: Brazil