Catechin-incorporated dental copolymers inhibit growth of Streptococcus mutans
J. appl. oral sci
;
21(2): 203-207, Mar-Apr/2013. tab, graf
Article
in English
| LILACS
| ID: lil-674359
ABSTRACT
Objective:
To test the inhibitory growth activity of green tea catechin incorporated into dental resins compared to resins containing the broad-spectrum antimicrobial compound chlorhexidine against Streptococcus mutans in vitro. Material andMethods:
The minimum inhibitory concentrations (MICs) of epigallocatechin-gallate (EGCg) and chlorhexidine (CHX) were determined according to the microdilution method. Resin discs (5 mm × 3 mm) were prepared from Bis-GMA/TEGDMA (R1) and Bis-GMA/CH3Bis-GMA (R2) comonomers (n=9) containing a) no drug, b) EGCg, c) CHX. Two concentrations of each drug (0.5× MIC and 1× MIC) were incorporated into the resin discs. Samples were individually immersed in a bacterial culture and incubated for 24 h at 37°C under constant agitation. Cell viability was assessed by counting the number of colonies on replica agar plates. Statistical analysis was performed using one-way ANOVA, Tukey and Student t-tests (α=0.05).Results:
Both resins containing EGCg and CHX showed a significant inhibition of bacterial growth at both concentrations tested (p<0.05). A significantly higher inhibition was observed in response to resins containing CHX at 0.5× MIC and 1× MIC, and EGCg at 1× MIC when compared to EGCg at 0.5× MIC. Also, EGCg at 0.5× MIC in R1 had a significantly higher growth inhibition than in R2.Conclusions:
Both EGCg and CHX retained their antibacterial activity when incorporated into the resin matrix. EGCg at 1× MIC in R1 and R2 resins significantly reduced S. mutans survival at a level similar to CHX. The data generated from this study will provide advances in the field of bioactive dental materials with the potential of improving the lifespan of resin-based restorations.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Streptococcus mutans
/
Catechin
/
Bisphenol A-Glycidyl Methacrylate
Limits:
Humans
Language:
English
Journal:
J. appl. oral sci
Journal subject:
Dentistry
Year:
2013
Type:
Article
Affiliation country:
Canada
Institution/Affiliation country:
University of Toronto/CA
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