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Human adenovirus detection among immunocompetent and immunocompromised patients presenting acute respiratory infection
Watanabe, Aripuana; Carraro, Emerson; Camargo, Clarice; Puerari, Diane; Guatura, Sandra; Granato, Celso; Bellei, Nancy.
  • Watanabe, Aripuana; Universidade Federal de São Paulo. Departamento de Medicina. Disciplina de Infectologia. Laboratório de Virologia Clínica. São Paulo. BR
  • Carraro, Emerson; Universidade Federal de São Paulo. Departamento de Medicina. Disciplina de Infectologia. Laboratório de Virologia Clínica. São Paulo. BR
  • Camargo, Clarice; Universidade Federal de São Paulo. Departamento de Medicina. Disciplina de Infectologia. Laboratório de Virologia Clínica. São Paulo. BR
  • Puerari, Diane; Universidade Federal de São Paulo. Departamento de Medicina. Disciplina de Infectologia. Laboratório de Virologia Clínica. São Paulo. BR
  • Guatura, Sandra; Universidade Federal de São Paulo. Departamento de Medicina. Disciplina de Infectologia. Laboratório de Virologia Clínica. São Paulo. BR
  • Granato, Celso; Universidade Federal de São Paulo. Departamento de Medicina. Disciplina de Infectologia. Laboratório de Virologia Clínica. São Paulo. BR
  • Bellei, Nancy; Universidade Federal de São Paulo. Departamento de Medicina. Disciplina de Infectologia. Laboratório de Virologia Clínica. São Paulo. BR
Rev. Soc. Bras. Med. Trop ; 46(2): 161-165, Mar-Apr/2013. tab, graf
Article in English | LILACS | ID: lil-674638
ABSTRACT

INTRODUCTION:

Human adenoviruses (HAdV) play an important role in the etiology of severe acute lower respiratory infection, especially in immunocompromised individuals. The aim of the present study was detect the HAdV through different

methods:

direct fluorescence assay (DFA) and nested-polymerase chain reaction (PCR-nested) from patients with acute respiratory infection (ARI) up to 7 days of symptoms onset.

METHODS:

Samples (n=643) were collected from different risk groups during from 2001 to 2010 139 adults attended in an Emergency Room Patients (ERP); 205 health care workers (HCW); 69 from Renal Transplant Outpatients (RTO); 230 patients in hematopoietic stem cell transplantation (HSCT) program.

RESULTS:

Among all patients (n=643) adenovirus was detected on 13.2% by DFA and/or PCR 6/139 (4.3%) adults from ERP, 7/205 (3.4%) from HCW samples, 4/69 (5.8%) from RTO and 68/230 (29.5%) from HSCT patients. Nested PCR showed higher detection (10%) compared to DFA test (3.8%) (p < 0.001). HSCT patients presented significantly higher prevalence of HAdV infection.

CONCLUSIONS:

Adenovirus detection through nested-PCR assay was higher. However the inclusion of molecular method in laboratorial routine diagnostic should be evaluated considering the reality of each specific health service. .
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Respiratory Tract Infections / Adenovirus Infections, Human Type of study: Diagnostic study / Prevalence study / Risk factors Limits: Adolescent / Adult / Aged / Aged80 / Child / Female / Humans / Male Country/Region as subject: South America / Brazil Language: English Journal: Rev. Soc. Bras. Med. Trop Journal subject: Tropical Medicine Year: 2013 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Respiratory Tract Infections / Adenovirus Infections, Human Type of study: Diagnostic study / Prevalence study / Risk factors Limits: Adolescent / Adult / Aged / Aged80 / Child / Female / Humans / Male Country/Region as subject: South America / Brazil Language: English Journal: Rev. Soc. Bras. Med. Trop Journal subject: Tropical Medicine Year: 2013 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de São Paulo/BR