Human adenovirus detection among immunocompetent and immunocompromised patients presenting acute respiratory infection
Rev. Soc. Bras. Med. Trop
;
46(2): 161-165, Mar-Apr/2013. tab, graf
Article
in English
| LILACS
| ID: lil-674638
ABSTRACT
INTRODUCTION:
Human adenoviruses (HAdV) play an important role in the etiology of severe acute lower respiratory infection, especially in immunocompromised individuals. The aim of the present study was detect the HAdV through differentmethods:
direct fluorescence assay (DFA) and nested-polymerase chain reaction (PCR-nested) from patients with acute respiratory infection (ARI) up to 7 days of symptoms onset.METHODS:
Samples (n=643) were collected from different risk groups during from 2001 to 2010 139 adults attended in an Emergency Room Patients (ERP); 205 health care workers (HCW); 69 from Renal Transplant Outpatients (RTO); 230 patients in hematopoietic stem cell transplantation (HSCT) program.RESULTS:
Among all patients (n=643) adenovirus was detected on 13.2% by DFA and/or PCR 6/139 (4.3%) adults from ERP, 7/205 (3.4%) from HCW samples, 4/69 (5.8%) from RTO and 68/230 (29.5%) from HSCT patients. Nested PCR showed higher detection (10%) compared to DFA test (3.8%) (p < 0.001). HSCT patients presented significantly higher prevalence of HAdV infection.CONCLUSIONS:
Adenovirus detection through nested-PCR assay was higher. However the inclusion of molecular method in laboratorial routine diagnostic should be evaluated considering the reality of each specific health service. .
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Respiratory Tract Infections
/
Adenovirus Infections, Human
Type of study:
Diagnostic study
/
Prevalence study
/
Risk factors
Limits:
Adolescent
/
Adult
/
Aged
/
Aged80
/
Child
/
Female
/
Humans
/
Male
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Rev. Soc. Bras. Med. Trop
Journal subject:
Tropical Medicine
Year:
2013
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal de São Paulo/BR
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