DsRed2 transient expression in Culex quinquefasciatus mosquitoes
Mem. Inst. Oswaldo Cruz
;
108(4): 529-531, jun. 2013. graf
Article
in English
| LILACS
| ID: lil-678292
ABSTRACT
Culex quinquefasciatus mosquitoes have been successfully genetically modified only once, despite the efforts of several laboratories to transform and establish a stable strain. We have developed a transient gene expression method, in Culex, that delivers plasmid DNA directly to the mosquito haemolymph and additional tissues. We were able to express DsRed2 fluorescent protein in adult Cx. quinquefasciatus mosquitoes by injecting plasmids directly into their thorax. The expression of DsRed2 in adult Cx. quinquefasciatus mosquitoes is an important stepping stone to genetic transformation and the potential use of new control strategies and genetic interactions.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Transformation, Genetic
/
Gene Expression
/
Culex
/
Insect Vectors
/
Luminescent Proteins
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2013
Type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade de Sao Paulo/BR
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