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Identification of microRNAs and mRNAs associated with multidrug resistance of human laryngeal cancer Hep-2 cells
Brazilian Journal of Medical and Biological Research; Yin, Wanzhong; Wang, Ping; Wang, Xin; Song, Wenzhi; Cui, Xiangyan; Yu, Hong; Zhu, Wei.
  • Yin, Wanzhong; Jilin University. The First Clinical Hospital, Norman Bethune College of Medicine. Head and Neck Surgery. Department of Otorhinolaryngology. Changchun. CN
  • Wang, Ping; Jilin University. The First Clinical Hospital, Norman Bethune College of Medicine. Head and Neck Surgery. Department of Otorhinolaryngology. Changchun. CN
  • Wang, Xin; Jilin University. The First Clinical Hospital, Norman Bethune College of Medicine. Head and Neck Surgery. Department of Otorhinolaryngology. Changchun. CN
  • Song, Wenzhi; Jilin University. The First Clinical Hospital, Norman Bethune College of Medicine. Head and Neck Surgery. Department of Otorhinolaryngology. Changchun. CN
  • Cui, Xiangyan; Jilin University. The First Clinical Hospital, Norman Bethune College of Medicine. Head and Neck Surgery. Department of Otorhinolaryngology. Changchun. CN
  • Yu, Hong; Jilin University. The First Clinical Hospital, Norman Bethune College of Medicine. Head and Neck Surgery. Department of Otorhinolaryngology. Changchun. CN
  • Zhu, Wei; Jilin University. The First Clinical Hospital, Norman Bethune College of Medicine. Head and Neck Surgery. Department of Otorhinolaryngology. Changchun. CN
Braz. j. med. biol. res ; 46(6): 546-554, 02/jul. 2013. tab, graf
Article in English | LILACS | ID: lil-679208
ABSTRACT
Multidrug resistance (MDR) poses a serious impediment to the success of chemotherapy for laryngeal cancer. To identify microRNAs and mRNAs associated with MDR of human laryngeal cancer Hep-2 cells, we developed a multidrug-resistant human laryngeal cancer subline, designated Hep-2/v, by exposing Hep-2 cells to stepwise increasing concentrations of vincristine (0.02-0.96'µM). Microarray assays were performed to compare the microRNA and mRNA expression profiles of Hep-2 and Hep-2/v cells. Compared to Hep-2 cells, Hep-2/v cells were more resistant to chemotherapy drugs (∼45-fold more resistant to vincristine, 5.1-fold more resistant to cisplatin, and 5.6-fold more resistant to 5-fluorouracil) and had a longer doubling time (42.33±1.76 vs 28.75±1.12'h, P<0.05), higher percentage of cells in G0/G1 phase (80.98±0.52 vs 69.14±0.89, P<0.05), increased efflux of rhodamine 123 (95.97±0.56 vs 12.40±0.44%, P<0.01), and up-regulated MDR1 expression. A total of 7 microRNAs and 605 mRNAs were differentially expressed between the two cell types. Of the differentially expressed mRNAs identified, regulator of G-protein signaling 10, high-temperature requirement protein A1, and nuclear protein 1 were found to be the putative targets of the differentially expressed microRNAs identified. These findings may open a new avenue for clarifying the mechanisms responsible for MDR in laryngeal cancer.
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Full text: Available Index: LILACS (Americas) Main subject: RNA, Messenger / Laryngeal Neoplasms / ATP Binding Cassette Transporter, Subfamily B, Member 1 / Drug Resistance, Neoplasm / MicroRNAs Type of study: Diagnostic study / Prognostic study / Risk factors Limits: Humans Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2013 Type: Article Affiliation country: China Institution/Affiliation country: Jilin University/CN

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Full text: Available Index: LILACS (Americas) Main subject: RNA, Messenger / Laryngeal Neoplasms / ATP Binding Cassette Transporter, Subfamily B, Member 1 / Drug Resistance, Neoplasm / MicroRNAs Type of study: Diagnostic study / Prognostic study / Risk factors Limits: Humans Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2013 Type: Article Affiliation country: China Institution/Affiliation country: Jilin University/CN