Monitoring Trypanosoma cruzi infection in triatomines using PCR in Mato Grosso do Sul, Brazil
Rev. Soc. Bras. Med. Trop
;
46(3): 277-280, May-Jun/2013. tab, graf
Article
in English
| LILACS
| ID: lil-679513
ABSTRACT
Introduction The aim of the present study was to assess the polymerase chain reaction (PCR) as a method for detecting Trypanosoma cruzi infection in triatomines that had been previously determined by microscopic examination in the State of Mato Grosso do Sul, Brazil. Methods In total, 515 specimens were collected. Material from the digestive tract of each triatomine was analyzed for the presence of T. cruzi by microscopic examination and PCR using the 121/122 primer set. Results Among the 515 specimens tested, 58 (11.3%) were positive by microscopy and 101 (19.61%) were positive by PCR and there was an association between the results of the techniques (χ2 = 53.354, p = 0.001). The main species of triatomine identified was T. sordida (95.5%) Conclusions The use of PCR in entomological surveillance may contribute to a better assessment of the occurrence of T. cruzi in triatomine populations. .
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Index:
LILACS (Americas)
Main subject:
Trypanosoma cruzi
/
Polymerase Chain Reaction
/
DNA, Protozoan
/
Triatominae
/
Insect Vectors
Limits:
Animals
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Rev. Soc. Bras. Med. Trop
Journal subject:
Tropical Medicine
Year:
2013
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal de Mato Grosso do Sul/BR
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