Analysis of mRNA expression profiles of carotenogenesis and astaxanthin production of Haematococcus pluvialis under exogenous 2, 4-epibrassinolide (EBR)
Biol. Res
;
46(2): 201-206, 2013. ilus, tab
Article
in English
| LILACS
| ID: lil-683998
ABSTRACT
The fresh-water green unicellular alga Haematococcus pluvialis is known to accumulate astaxanthin under stress conditions. In the present study, transcriptional expression of eight genes involved in astaxanthin biosynthesis exposed to EBR (25 and 50 mg/L) was analyzed using qRT-PCR. The results demonstrated that both 25 and 50 mg/L EBR could increase astaxanthin productivity and the eight carotenogenic genes were up-regulated by EBR with different expression profiles. Moreover, EBR25 induction had a greater influence on the transcriptional expression of ipi-1, ipi-2, crtR-B, lyc and crtO (> 5- fold up-regulation) than on psy, pds, bkt; EBR50 treatment had a greater effect on the transcriptional expression of ipi-2, pds, lyc, crtR-B, bkt and crtO than on ipi-1 and psy. Furthermore, astaxanthin biosynthesis under EBR was up-regulated mainly by ipi1־ and psy at the post-transcriptional level, pds, lyc, crtR-B, bkt and crtO at the transcriptional level and ipi-2 at both levels.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Plant Growth Regulators
/
Steroids, Heterocyclic
/
RNA, Messenger
/
Carotenoids
/
Chlorophyta
/
Brassinosteroids
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
2013
Type:
Article
/
Project document
Affiliation country:
China
Institution/Affiliation country:
Shandong University of Technology/CN
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