3-D modeling and molecular dynamics simulation of interleukin-22 from the So-iny mullet, Liza haematocheila
Electron. j. biotechnol
; 16(4): 3-3, July 2013. ilus, tab
Article
in En
| LILACS
| ID: lil-684018
Responsible library:
CL1.1
ABSTRACT
Background:
Interleukin-22 (IL-22) plays an important role in the regulation of immune responses. However, little is known about its function or structure in fish.Results:
The IL-22 gene was first cloned from So-iny mullet (Liza haematocheila), one of commercially important fish species in China. Then, 3-D structure model of the mullet IL-22 was constructed by comparative modeling method using human IL-22 (1M4R) as template, and a 5 ns molecular dynamics (MD) was studied. The open reading frame (ORF) of mullet IL-22 cDNA was 555 bp, encoding 184 amino acids. The mullet IL-22 shared higher identities with the other fish IL-22 homologs and possessed a conserved IL-10 signature motif at its C-terminal. The mullet IL-22 model possessed six conserved helix structure. PROCHECK, SAVES and Molprobity server analysis confirmed that this model threaded well with human IL-22. Strikingly, analysis with CastP, cons-PPISP server suggested that the cysteines in mullet IL-22 might not be involved in the forming of disulfide bond for structural stabilization, but related to protein-protein interactions.Conclusions:
The structure of IL-22 in So-iny mullet (Liza haematocheila) was constructed using comparative modeling method which provide more information for studying the function of fish IL-22.Key words
Full text:
1
Index:
LILACS
Main subject:
Interleukins
/
Molecular Dynamics Simulation
/
Fishes
Limits:
Animals
Language:
En
Journal:
Electron. j. biotechnol
Journal subject:
BIOTECNOLOGIA
Year:
2013
Type:
Article