Your browser doesn't support javascript.
loading
Effect of tramadol on lung injury induced by skeletal muscle ischemia-reperfusion: an experimental study / Efeito do tramadol na lesao pulmonar induzida por isquemia-reperfusao de musculo esqueletico: um estudo experimental
Takhtfooladi, Mohammad Ashrafzadeh; Jahanshahi, Amirali; Sotoudeh, Amir; Jahanshahi, Gholamreza; Takhtfooladi, Hamed Ashrafzadeh; Aslani, Kimia.
  • Takhtfooladi, Mohammad Ashrafzadeh; s.af
  • Jahanshahi, Amirali; s.af
  • Sotoudeh, Amir; s.af
  • Jahanshahi, Gholamreza; s.af
  • Takhtfooladi, Hamed Ashrafzadeh; s.af
  • Aslani, Kimia; s.af
J. bras. pneumol ; 39(4): 434-439, June-August/2013. graf
Article in English | LILACS | ID: lil-686607
ABSTRACT

OBJECTIVE:

To determine whether tramadol has a protective effect against lung injury induced by skeletal muscle ischemia-reperfusion.

METHODS:

Twenty Wistar male rats were allocated to one of two groups ischemia-reperfusion (IR) and ischemia-reperfusion + tramadol (IR+T). The animals were anesthetized with intramuscular injections of ketamine and xylazine (50 mg/kg and 10 mg/kg, respectively). All of the animals underwent 2-h ischemia by occlusion of the femoral artery and 24-h reperfusion. Prior to the occlusion of the femoral artery, 250 IU heparin were administered via the jugular vein in order to prevent clotting. The rats in the IR+T group were treated with tramadol (20 mg/kg i.v.) immediately before reperfusion. After the reperfusion period, the animals were euthanized with pentobarbital (300 mg/kg i.p.), the lungs were carefully removed, and specimens were properly prepared for histopathological and biochemical studies.

RESULTS:

Myeloperoxidase activity and nitric oxide levels were significantly higher in the IR group than in the IR+T group (p = 0.001 for both). Histological abnormalities, such as intra-alveolar edema, intra-alveolar hemorrhage, and neutrophil infiltration, were significantly more common in the IR group than in the IR+T group.

CONCLUSIONS:

On the basis of our histological and biochemical findings, we conclude that tramadol prevents lung tissue injury after skeletal muscle ischemia-reperfusion. .
RESUMO

OBJETIVO:

Investigar se o tramadol tem um efeito protetor contra a lesão pulmonar induzida por isquemia-reperfusão de músculo esquelético.

MÉTODOS:

Vinte ratos Wistar machos foram divididos em dois grupos grupo isquemia-reperfusão (IR) e grupo isquemia-reperfusão + tramadol (IR+T). Os animais foram anestesiados com cetamina e xilazina (i.m., 50 mg/kg e 10 mg/kg, respectivamente). Todos os animais foram submetidos a 2 h de isquemia por oclusão da artéria femoral e 24 h de reperfusão. Antes da oclusão da artéria femoral, foram administrados 250 UI de heparina pela veia jugular para impedir a coagulação. Os ratos do grupo IR+T foram tratados com tramadol (20 mg/kg i.v.) imediatamente antes da reperfusão. Após o período de reperfusão, os animais foram sacrificados com pentobarbital (300 mg/kg i.p.), os pulmões foram removidos cuidadosamente, e os espécimes foram preparados adequadamente para estudos histopatológicos e bioquímicos.

RESULTADOS:

A atividade de mieloperoxidase e os níveis de óxido nítrico foram significativamente maiores no grupo IR que no grupo IR+T (p = 0,001 para ambos). Anormalidades histológicas, como edema intra-alveolar, hemorragia intra-alveolar e infiltração neutrofílica, foram significativamente mais frequentes no grupo IR que no grupo IR+T.

CONCLUSÕES:

Com base nos resultados histológicos e bioquímicos ...
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Tramadol / Reperfusion Injury / Muscle, Skeletal / Lung Injury / Lung Type of study: Etiology study / Prognostic study Limits: Animals Language: English Journal: J. bras. pneumol Journal subject: Pulmonary Disease (Specialty) Year: 2013 Type: Article

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Index: LILACS (Americas) Main subject: Tramadol / Reperfusion Injury / Muscle, Skeletal / Lung Injury / Lung Type of study: Etiology study / Prognostic study Limits: Animals Language: English Journal: J. bras. pneumol Journal subject: Pulmonary Disease (Specialty) Year: 2013 Type: Article