Validation of new analytical methodology for determining fenoterolhydrobromide by HPLC: application in pharmaceutical products
Rev. Inst. Adolfo Lutz
;
71(2): 355-361, abr.-jun. 2012. tab, graf
Article
in English
| LILACS, SES-SP, SESSP-CTDPROD, SES-SP, SESSP-ACVSES, SESSP-IALPROD, SES-SP, SESSP-IALACERVO
| ID: lil-688207
ABSTRACT
Fenoterol hydrobromide is a B2-adrenergic agonist agent used for asthma and chronic obstructive pulmonary disease treatment. HPLC methodology was developed and validated for quantitative determination of fenoterol hydrobromide. The methodology was achieved by using a reversed-phase C18column, (150 mm ¡Á 3.9 mm i.d., 5 ¦Ìm) Thermo. The mobile phase was consisted of acetonitrile water(3070, v/v) with 0,1% triethylamine, pH adjusted to 5.0 with formic acid and flow rate of 1.0 mL.min-1with UV detection at 276 nm. The concentration range was from 0.025 to 0.15 mg.mL-1, and the correlation coefficient of analytical curve was >0.999. The detection limit and the quantifying limit (QL) were 0.003mg.mL-1 and 0.012 mg.mL-1, respectively. Intra- and interday relative standard deviations were ¡Ü2.0%. Themetho dology accuracy showed the percentage mean of 99.53%. The described technique was found to be simple, rapid, precise, accurate and sensitive; the advantages over the others current methodologies arethe low-cost and low-polluting conditions. Owing to its simplicity and reliable results, this methodology is suitable to be used in quality control of pharmaceutical drugs containing fenoterol hydrobromide as active componente.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Hyoscyaminum Bromatum
/
Chromatography, Liquid
/
Pharmaceutical Raw Material
/
Exercise Test
/
Fenoterol
Language:
English
Journal:
Rev. Inst. Adolfo Lutz
Year:
2012
Type:
Article
Institution/Affiliation country:
Instituto Adolfo Lutz/BR
/
Universidade de São Paulo/BR
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