Your browser doesn't support javascript.
loading
Myxovirus resistance, osteopontin and suppressor of cytokine signaling 3 polymorphisms predict hepatitis C virus therapy response in an admixed patient population: comparison with IL28B
Angelo, Ana Luiza Dias; Cavalcante, Lourianne Nascimento; Abe-Sandes, Kiyoko; Machado, Taisa Bonfim; Lemaire, Denise Carneiro; Malta, Fernanda; Pinho, Joao Renato; Lyra, Luiz Guilherme Costa; Lyra, Andre Castro.
  • Angelo, Ana Luiza Dias; Federal University of Bahia. Laboratory of Immunology. Salvador. BR
  • Cavalcante, Lourianne Nascimento; Federal University of Bahia. Laboratory of Immunology. Salvador. BR
  • Abe-Sandes, Kiyoko; Federal University of Bahia. Laboratory of Immunology. Salvador. BR
  • Machado, Taisa Bonfim; Federal University of Bahia. Laboratory of Immunology. Salvador. BR
  • Lemaire, Denise Carneiro; Federal University of Bahia. Laboratory of Immunology. Salvador. BR
  • Malta, Fernanda; Federal University of Bahia. Laboratory of Immunology. Salvador. BR
  • Pinho, Joao Renato; Federal University of Bahia. Laboratory of Immunology. Salvador. BR
  • Lyra, Luiz Guilherme Costa; Federal University of Bahia. Laboratory of Immunology. Salvador. BR
  • Lyra, Andre Castro; Federal University of Bahia. Laboratory of Immunology. Salvador. BR
Clinics ; 68(10): 1325-1332, out. 2013. tab, graf
Article in English | LILACS | ID: lil-689983
ABSTRACT

OBJECTIVES:

Suppressor of cytokine signaling 3, myxovirus resistance protein and osteopontin gene polymorphisms may influence the therapeutic response in patients with chronic hepatitis C, and an association with IL28 might increase the power to predict sustained virologic response. Our aims were to evaluate the association between myxovirus resistance protein, osteopontin and suppressor of cytokine signaling 3 gene polymorphisms in combination with IL28B and to assess the therapy response in hepatitis C patients treated with pegylated-interferon plus ribavirin.

METHOD:

Myxovirus resistance protein, osteopontin, suppressor of cytokine signaling 3 and IL28B polymorphisms were analyzed by PCR-restriction fragment length polymorphism, direct sequencing and real-time PCR. Ancestry was determined using genetic markers.

RESULTS:

We analyzed 181 individuals, including 52 who were sustained virologic responders. The protective genotype frequencies among the sustained virologic response group were as follows the G/G suppressor of cytokine signaling 3 (rs4969170) (62.2%); T/T osteopontin (rs2853744) (60%); T/T osteopontin (rs11730582) (64.3%); and the G/T myxovirus resistance protein (rs2071430) genotype (54%). The patients who had ≥3 of the protective genotypes from the myxovirus resistance protein, the suppressor of cytokine signaling 3 and osteopontin had a greater than 90% probability of achieving a sustained response (p<0.0001). The C/C IL28B genotype was present in 58.8% of the subjects in this group. The sustained virological response rates increased to 85.7% and 91.7% by analyzing C/C IL28B with the T/T osteopontin genotype at rs11730582 and the G/G suppressor of cytokine signaling 3 genotype, respectively. Genetic ancestry analysis revealed an admixed population.

CONCLUSION:

Hepatitis C genotype 1 patients who were responders to interferon-based therapy had a high frequency of multiple protective polymorphisms in the myxovirus ...
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Polymorphism, Genetic / Interleukins / Hepatitis C, Chronic / Suppressor of Cytokine Signaling Proteins / Osteopontin / Myxovirus Resistance Proteins Type of study: Evaluation studies / Prognostic study / Risk factors Limits: Adult / Female / Humans / Male Language: English Journal: Clinics Journal subject: Medicine Year: 2013 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Federal University of Bahia/BR

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Index: LILACS (Americas) Main subject: Polymorphism, Genetic / Interleukins / Hepatitis C, Chronic / Suppressor of Cytokine Signaling Proteins / Osteopontin / Myxovirus Resistance Proteins Type of study: Evaluation studies / Prognostic study / Risk factors Limits: Adult / Female / Humans / Male Language: English Journal: Clinics Journal subject: Medicine Year: 2013 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Federal University of Bahia/BR