Comparison between two methods for molecular characterization of human papillomavirus
DST j. bras. doenças sex. transm
;
25(1)2013.
Article
in English
| LILACS
| ID: lil-696423
ABSTRACT
A sensitive method of detection for Human Papillomavirus (HPV) is important to facilitate the early treatment of cervical cancer precursors.Objective:
to analyze the spectrum of HPV infection and compare the sensibility of DNA HPV detection using polymerase chain reaction (PCR) and nestedPCR (nPCR) methods in a group of 251 women of Pelotas-RS.Methods:
genomic DNA was extracted from the collected samples and was submitted toPCR methods with the primers MY09/11 and nPCR with the pair of primers MY09/MY11 and GP5+/6+. The results were applied to the softwares Epi-Info v.3.5.1* and STATA v.11 * for analyzes.Results:
the prevalence of HPV infection was 6.8% with the use of primers MY09/11. When associated withprimers GP5/6, this result increased to 29.9% (p < 0.001).Conclusion:
the increase founded in HPV DNA detection from 6.8 to 29.9% suggests that thetechnique of nPCR MY09/11 followed by GP5/6 is the most sensitive method to detect HPV DNA from cervical specimens.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Papillomaviridae
/
DNA Probes, HPV
/
Polymerase Chain Reaction
/
Molecular Diagnostic Techniques
Type of study:
Diagnostic study
/
Observational study
/
Prevalence study
/
Risk factors
Limits:
Adolescent
/
Female
/
Humans
Language:
English
Journal:
DST j. bras. doenças sex. transm
Journal subject:
Sexually Transmitted Diseases
Year:
2013
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Federal University of Pelotas/BR
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