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Effect of cryopreservation on the efficiency of exogenous gene, genetic transformation and expression level of Arabidopsis thaliana
Li, Zhongai; Du, Yaqiong; Wang, Zicheng.
  • Li, Zhongai; Henan University. College of Life Science. Plant Germplasm Resources and Genetic Laboratory. Kaifeng. CN
  • Du, Yaqiong; Henan University. College of Life Science. Plant Germplasm Resources and Genetic Laboratory. Kaifeng. CN
  • Wang, Zicheng; Henan University. College of Life Science. Plant Germplasm Resources and Genetic Laboratory. Kaifeng. CN
Electron. j. biotechnol ; 16(6): 12-12, Nov. 2013. ilus, tab
Article in English | LILACS | ID: lil-696553
ABSTRACT

Background:

Cryopreservation refers to the storage of a living organism at ultra-low-temperature for long-term preservation of plant germplasm. The effect of cryopreservation on the efficiency of exogenous gene genetic transformation and expression level were studied herein. In this work, transgenic Arabidopsis thaliana were successfully conserved in vitro by cryopreservation methods.

Results:

The effects of osmotic stress due to cryoprotectants during pretreatment and of storage at -196ºC on the stability, the efficiency of genetic transformation and the expression level of exogenous gene were analyzed in Arabidopsis. The results showed that there had not any significant increasing in the efficiency of genetic transformation after cryopreservation, and our observation was not in agreement with earlier reports. Transgenic Arabidopsis lines over-expressing ATOST1 gene were used for the real-time PCR analysis, and the result indicated that the expression of the ATOST1 gene was up-regulated about 2.4-fold in the transgenic seedlings tissues retrieved from cryopreservation than those non-cryopreserved counterparts. Conclusions: Cryopreservation could improve the expression of exogenous gene, however, could not promote the genetic transformation obviously.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Cryopreservation / Arabidopsis Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2013 Type: Article Affiliation country: China Institution/Affiliation country: Henan University/CN

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Full text: Available Index: LILACS (Americas) Main subject: Cryopreservation / Arabidopsis Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2013 Type: Article Affiliation country: China Institution/Affiliation country: Henan University/CN