Your browser doesn't support javascript.
loading
Isolation and purification of bromelain from waste peel of pineapple for therapeutic application
Bresolin, Iara Rocha Antunes Pereira; Bresolin, Igor Tadeu Lazzarotto; Silveira, Edgar; Tambourgi, Elias Basile; Mazzola, Priscila Gava.
  • Bresolin, Iara Rocha Antunes Pereira; Universidade Estadual de Campinas. Faculdade de Engenharia Química. Campinas. BR
  • Bresolin, Igor Tadeu Lazzarotto; Universidade Estadual de Campinas. Faculdade de Engenharia Química. Campinas. BR
  • Silveira, Edgar; Universidade Estadual de Campinas. Faculdade de Engenharia Química. Campinas. BR
  • Tambourgi, Elias Basile; Universidade Estadual de Campinas. Faculdade de Engenharia Química. Campinas. BR
  • Mazzola, Priscila Gava; Universidade Estadual de Campinas. Faculdade de Engenharia Química. Campinas. BR
Braz. arch. biol. technol ; 56(6): 971-979, Nov.-Dec. 2013. ilus, tab
Article in English | LILACS | ID: lil-696944
ABSTRACT
The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, maintaining its enzymatic activity. The batch adsorption of bromelain was evaluated in terms of total protein and enzymatic activity using Langmuir and Langmuir-Freundlich models. Results showed that the process could be suitable for the recovery and purification of the enzyme, maintaining its specific activity.


Full text: Available Index: LILACS (Americas) Language: English Journal: Braz. arch. biol. technol Journal subject: Biology Year: 2013 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual de Campinas/BR

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Index: LILACS (Americas) Language: English Journal: Braz. arch. biol. technol Journal subject: Biology Year: 2013 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual de Campinas/BR