A simple, rapid and economic method for detecting multidrug-resistant tuberculosis
Braz. j. infect. dis
;
17(6): 667-671, Nov.-Dec. 2013. ilus, tab
Article
in English
| LILACS
| ID: lil-696968
ABSTRACT
OBJECTIVE: To evaluate multiplex allele specific polymerase chain reaction as a rapid molecular tool for detecting multidrug-resistant tuberculosis. METHODS: Based on drug susceptibility testing, 103 isolates were multidrug-resistant tuberculosis and 45 isolates were sensitive to isonicotinylhydrazine and rifampin. Primers were designed to target five mutations hotspots that confer resistance to the first-line drugs isoniazid and rifampin, and multiplex allele specific polymerase chain reaction was performed. Whole-genome sequencing confirmed drug resistance mutations identified by multiplex allele specific polymerase chain reaction. RESULTS: DNA sequencing revealed that 68.9% of multidrug-resistant strains have point mutations at codon 315 of the katG gene, 19.8% within the mabA-inhA promoter, and 98.0% at three hotspots within rpoB. Multiplex allele specific polymerase chain reaction detected each of these five mutations, yielding 82.3% sensitivity and 100% specificity for isoniazid resistance, and 97.9% sensitivity and 100% specificity for rifampin resistance as compared to drug susceptibility testing. CONCLUSIONS: The results show that multiplex allele specific polymerase chain reaction is an inexpensive and practical method for rapid detection of multidrug-resistant tuberculosis in developing countries.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Tuberculosis, Multidrug-Resistant
/
Multiplex Polymerase Chain Reaction
/
Mycobacterium tuberculosis
/
Antitubercular Agents
Type of study:
Diagnostic study
/
Health economic evaluation
/
Prognostic study
Limits:
Humans
Language:
English
Journal:
Braz. j. infect. dis
Journal subject:
Communicable Diseases
Year:
2013
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
Xinxiang Medical University/CN
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