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A conventional polymerase chain reaction-based method for the diagnosis of human schistosomiasis in stool samples from individuals in a low-endemicity area
Carneiro, Teiliane Rodrigues; Peralta, Regina Helena Saramago; Pinheiro, Marta Cristhiany Cunha; Oliveira, Sara Menezes de; Peralta, Jose Mauro; Bezerra, Fernando Schemelzer Moraes.
  • Carneiro, Teiliane Rodrigues; Universidade Federal do Ceara. Departamento de Patologia e Medicina Legal. Fortaleza. BR
  • Peralta, Regina Helena Saramago; Universidade Federal do Ceara. Departamento de Patologia e Medicina Legal. Fortaleza. BR
  • Pinheiro, Marta Cristhiany Cunha; Universidade Federal do Ceara. Departamento de Patologia e Medicina Legal. Fortaleza. BR
  • Oliveira, Sara Menezes de; Universidade Federal do Ceara. Departamento de Patologia e Medicina Legal. Fortaleza. BR
  • Peralta, Jose Mauro; Universidade Federal do Ceara. Departamento de Patologia e Medicina Legal. Fortaleza. BR
  • Bezerra, Fernando Schemelzer Moraes; Universidade Federal do Ceara. Departamento de Patologia e Medicina Legal. Fortaleza. BR
Mem. Inst. Oswaldo Cruz ; 108(8): 1037-1044, 6/dez. 2013. tab
Article in English | LILACS | ID: lil-697144
Responsible library: BR1.1
ABSTRACT
The aim of this study was to evaluate the efficacy of a polymerase chain reaction (PCR)-based method to detect Schistosoma mansoni DNA in stool samples from individuals living in a low-endemicity area in Brazil. Of the 125 initial stool samples, 80 were ELISA reactive and eggs were identified in 19 of the samples by parasitological examination. For the PCR evaluations, 56 stool samples were selected and divided into five groups. Groups I-IV were scored negative for S. mansoni eggs by parasitological examination. Groups I and II were ELISA reactive, whereas Groups III and IV were ELISA nonreactive. Groups II and III were positive for other intestinal parasites. PCR testing scored eight samples as positive from these four groups. Group V represented the S. mansoni -positive group and it included ELISA-reactive samples that were scored positive for S. mansoni by one or more parasitological examinations (6/19 were positive by Kato-Katz method, 9/17 by saline gradient and 10/13 by Helmintex®). PCR scored 13 of these 19 samples as positive for S. mansoni . We conclude that while none of these methods yielded 100% sensitivity, a combination of techniques should be effective for improving the detection of S. mansoni infection in low-endemicity areas.
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Full text: Available Index: LILACS (Americas) Main subject: Schistosoma mansoni / Schistosomiasis mansoni / DNA, Helminth / Feces Limits: Animals / Humans Country/Region as subject: South America / Brazil Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2013 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal do Ceara/BR

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Full text: Available Index: LILACS (Americas) Main subject: Schistosoma mansoni / Schistosomiasis mansoni / DNA, Helminth / Feces Limits: Animals / Humans Country/Region as subject: South America / Brazil Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2013 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal do Ceara/BR