The testis of the mice C57/BL6 offspring in adulthood have alterations due to maternal caffeine consumption
Acta cir. bras
;
29(1): 16-23, 01/2014. tab, graf
Article
in English
| LILACS
| ID: lil-697560
ABSTRACT
To investigate the effects of the maternal caffeine consumption during pregnancy to adult male testis mice offspring. METHODS:
Twenty pregnant mice were divided into control group (c) and caffeine group (cf). dams received daily saline or 20 mg/kg of caffeine subcutaneously. Male offspring were monitored daily until 13th week. The testis were used to evaluate both the proliferation (pcna) and apoptosis (bax); leptin receptor (ob-r); aromatase; follicle stimulating hormone (fshr), luteinizing hormone (lhr) and androgen receptors (ar); steroidogenic acute regulatory protein (star); vascular endothelial growth factor (vegf) and estrogen receptors (erα and erβ) by western blotting. Serum concentrations of testosterone, estradiol and leptin were measured.RESULTS:
There was a significant reduction in food intake and the body mass gain (p<0.05) in the cf ; pcna (p=0.01), fshr (p=0.02), star (p=0.0007), vegf (p=0.009), ar (p=0.03) in the cf. while an increase were note in bax (p=0.01), ob-r (p=0.02), lhr (p=0.04) and in the aromatase (p=0.03) in the cf. only erα and erβ were not changed by maternal caffeine. The serum testosterone levels in the cf offspring were 90% lower than in the c offspring (p=0.04).CONCLUSION:
Maternal caffeine consumption has a role and alters the testis of the offspring in adulthood.
Full text:
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Index:
LILACS (Americas)
Main subject:
Caffeine
/
Pregnancy, Animal
/
Embryonic Development
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2014
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Estadual do Rio de Janeiro/BR
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