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Osteocalcin Immunohistochemical Expression During Repair of Critical-Sized Bone Defects Treated with Subcutaneous Adipose Tissue in Rat and Rabbit Animal Model
Verbicaro, Thalyta; Giovanini, Allan Fernando; Zielak, Joao Cesar; Baratto Filho, Flares; Araujo, Melissa Rodrigues de; Deliberador, Tatiana Miranda.
  • Verbicaro, Thalyta; Positivo University. Curitiba. BR
  • Giovanini, Allan Fernando; Positivo University. Curitiba. BR
  • Zielak, Joao Cesar; Positivo University. Curitiba. BR
  • Baratto Filho, Flares; Positivo University. Curitiba. BR
  • Araujo, Melissa Rodrigues de; Positivo University. Curitiba. BR
  • Deliberador, Tatiana Miranda; Positivo University. Curitiba. BR
Braz. dent. j ; 24(6): 559-564, Nov-Dec/2013. tab, graf
Article in English | LILACS | ID: lil-697666
ABSTRACT
The aim of this study was to evaluate the osteocalcin immunoexpression during the bone repair treated with subcutaneous adipose tissue in rats and rabbits. Fourteen rats and 14 rabbits were used in the study. A critical-sized calvarial defect was created in each animal. The animals were divided into 4 experimental groups RC (rat control), RbC (rabbit control), RAT (rat adipose tissue), RbAT (rabbit adipose tissue). In the groups RC and RbC the defect was filled with a blood clot. In groups RAT and RbAT, the defect was filled with macerated subcutaneous adipose tissue. The euthanasia was performed at 30 days (RC and RAT) and 40 days (RbC and RbAT). Histological analysis and immunohistochemical analysis of osteocalcin protein expression were performed. Data were submitted to descriptive statistical analysis (mode). Osteocalcin immunoexpression was detected in the experimental models. Notwithstanding, RbAT showed higher positivity, especially in the adipocytes, than the group RAT group. In groups RC and RbC, the surgical wound was filled by collagen fibers. In Group RAT, the defect was composed by collagen fibers and adipocytes in the reparative sites. Similarly, in RbAT, the defect also was partially filled by collagen fibers and presence of adipocytes dispersed among the fibers. Additionally, chronic inflammatory process and areas of bone matrix deposition were observed. It may be concluded that in both animal models the adipose tissue demonstrated low osteogenic capacity. However, the rabbit animal model exhibited a more evident osteocalcin immunoexpression and a greater amount of newly formed bone matrix.
RESUMO
Este estudo avaliou a imunoexpressão da osteocalcina no reparo ósseo de defeitos críticos tratados com tecido adiposo subcutâneo em rato e coelho. Foram utilizados 14 ratos e 14 coelhos. Um defeito crítico foi realizado na calvária de cada animal. Os animais foram divididos em 4 grupos experimentais CR (controle rato), CC (controle coelho), TAR (tecido adiposo do rato), TAC (tecido adiposo do coelho). Nos grupos CR e CC, o defeito foi preenchido com coágulo. Nos grupos TAR e TAC, o defeito foi preenchido com tecido adiposo subcutâneo macerado. A eutanásia dos grupos CR e TAR ocorreu com 30 dias e dos grupos CC e TAC com 40 dias. Foram realizadas análise histológica e imunoistoquímica com a proteína osteocalcina. Os dados foram submetidos à análise estatística descritiva (moda). Nos modelos experimentais foi observada a imunoexpressão de osteocalcina. Contudo, o TAC apresentou maior imunoexpressão entre os adipócitos quando comparado ao TAR. Nos grupos CR e CC a ferida cirúrgica foi preenchida por fibras colágenas. No grupo TAR foi observado o preenchimento por fibras colágenas e presença de adipócitos no interior deste tecido. No grupo TAC o defeito foi preenchido parcialmente por fibras colágenas, com presença de adipócitos dispersos entre as fibras. Além disso, foi observado processo inflamatório crônico e sinais de neoformação óssea. Conclui-se que em ambos os modelos animais o enxerto de tecido adiposo apresentou baixa capacidade osteogênica. Contudo, o modelo animal coelho apresentou uma imunoexpressão da osteocalcina mais evidente e maior quantidade de matriz óssea neoformada.
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Full text: Available Index: LILACS (Americas) Main subject: Skull / Wound Healing / Osteocalcin / Subcutaneous Fat Limits: Animals Language: English Journal: Braz. dent. j Journal subject: Dentistry Year: 2013 Type: Article Affiliation country: Brazil Institution/Affiliation country: Positivo University/BR

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Full text: Available Index: LILACS (Americas) Main subject: Skull / Wound Healing / Osteocalcin / Subcutaneous Fat Limits: Animals Language: English Journal: Braz. dent. j Journal subject: Dentistry Year: 2013 Type: Article Affiliation country: Brazil Institution/Affiliation country: Positivo University/BR