Alterations in the duodenum myenteric neurons of Wistar ratsafter ingesting of 2, 4 dichlorophenoxyacetic acid
Braz. j. morphol. sci
;
30(1): 28-32, 2013. tab, ilus
Article
in English
| LILACS
| ID: lil-699325
ABSTRACT
The 2,4 dichlorophenoxyacetic acid (2,4-D) is a systemic herbicide whose effects in animal organic systemshave been examined in previous studies, being the neurotoxicity considered the predominant effect. However,the studies that detect the 2,4-D neurotoxicity have merely focused in the central nervous system, andtherefore, little is known about the effect of this herbicide in the enteric nervous system. This study aimedto verifying the 2,4-D effects on the myenteric neurons in duodenum of Wistar rats. Ten 60-day-old maleWistar rats (Rattus norvegicus) were divided in two groups control group (C) that did not receive 2,4-D andexperimental group (E) that received 5.0 mg of 2,4-D/kg for 15 days. At the end of experimental period, theanimal were euthanized, the duodenum was collected and processed for NADPH-diaphorase histochemicalanalysis in order to expose the nitrergic myenteric neurons (NADPH-dp). In the light microscopy analysis, thewhole-mount preparation obtained from duodenum of each animal were image-captured in 120 and 40 fields,for quantitative and morphometric analyses of myenteric neurons, respectively. The neuronal density was notaffected when comparing the two groups, but an increase (p > 0.05) of 8.5% was observed in the cell bodyarea of neurons in the E group. In conclusion, the ingestion of 2,4-D at a dosage of 5.0 mg/kg body weightfor 15 days does not change the neuronal density, but promotes the hypertrophy of NADPH-dp myentericneurons in duodenum of the rats of this study.
Full text:
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Index:
LILACS (Americas)
Main subject:
Nitrergic Neurons
/
Herbicides
/
Intestine, Small
/
Myenteric Plexus
/
NADPH Dehydrogenase
Limits:
Animals
Language:
English
Journal:
Braz. j. morphol. sci
Journal subject:
Anatomy
Year:
2013
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Paranaense University/BR
/
Paulista State University/BR
/
State University of Maringá/BR
/
São Paulo University/BR
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