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Mutación del gen KRAS en el cáncer de colon y recto / KRAS gene mutation in colorectal cancer
Roa, Iván; Sánchez, Tamara; Majlis, Alejandro; Schalper, Kurt.
  • Roa, Iván; Creative Bioscience. Santiago. CL
  • Sánchez, Tamara; Creative Bioscience. Santiago. CL
  • Majlis, Alejandro; Creative Bioscience. Santiago. CL
  • Schalper, Kurt; Creative Bioscience. Santiago. CL
Rev. méd. Chile ; 141(9): 1166-1172, set. 2013. ilus, graf, tab
Article in Spanish | LILACS | ID: lil-699684
ABSTRACT

Background:

KRAS oncogene is involved in colorectal carcinogenesis in 22 to 45% of cases.

Aim:

To determine the frequency, types and distribution of KRAS mutations in colorectal cancer. Material and

Methods:

KRAS mutations studies were carried out in primary tumors and metastases of colo-rectal cancer from 56 women aged 60 ± 14 years and 53 men aged 61 ± 11 years. Formalin fixed and paraffin embedded tissue samples were evaluated using RFLP (Restriction Fragment Length Polymorphism) and direct sequencing.

Results:

Primary tumors were located in the colon and rectum in 82 (75.2%) and 24 cases (20%), respectively. In three cases the extraction site of the tumor sample was unknown. In 46 cases (42.2%) KRAS mutations were demonstrated. The main point mutations were located in codon 12 (80.4%), G12D (39.1%), G12V (24.2%), G12S (6.5%), G12A (4.3%); G12C (4.3%), G12R (2.1%) and 19.6% at codon 13 (G13D). No differences were demonstrated in the frequency and distribution of mutations by gender, age, primary versus metastatic tumors or tumor location.

Conclusions:

In this series, 42% of colorectal cancer tissue samples had KRAS mutations. Their frequency and distribution are similar to those reported in the literature, except for G12C mutation.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Rectal Neoplasms / Adenocarcinoma / Proto-Oncogene Proteins / Colonic Neoplasms / Ras Proteins Limits: Adult / Aged / Female / Humans / Male Country/Region as subject: South America / Chile Language: Spanish Journal: Rev. méd. Chile Journal subject: Medicine Year: 2013 Type: Article Affiliation country: Chile Institution/Affiliation country: Creative Bioscience/CL

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Full text: Available Index: LILACS (Americas) Main subject: Rectal Neoplasms / Adenocarcinoma / Proto-Oncogene Proteins / Colonic Neoplasms / Ras Proteins Limits: Adult / Aged / Female / Humans / Male Country/Region as subject: South America / Chile Language: Spanish Journal: Rev. méd. Chile Journal subject: Medicine Year: 2013 Type: Article Affiliation country: Chile Institution/Affiliation country: Creative Bioscience/CL