Mutación del gen KRAS en el cáncer de colon y recto / KRAS gene mutation in colorectal cancer
Rev. méd. Chile
;
141(9): 1166-1172, set. 2013. ilus, graf, tab
Article
in Spanish
| LILACS
| ID: lil-699684
ABSTRACT
Background:
KRAS oncogene is involved in colorectal carcinogenesis in 22 to 45% of cases.Aim:
To determine the frequency, types and distribution of KRAS mutations in colorectal cancer. Material andMethods:
KRAS mutations studies were carried out in primary tumors and metastases of colo-rectal cancer from 56 women aged 60 ± 14 years and 53 men aged 61 ± 11 years. Formalin fixed and paraffin embedded tissue samples were evaluated using RFLP (Restriction Fragment Length Polymorphism) and direct sequencing.Results:
Primary tumors were located in the colon and rectum in 82 (75.2%) and 24 cases (20%), respectively. In three cases the extraction site of the tumor sample was unknown. In 46 cases (42.2%) KRAS mutations were demonstrated. The main point mutations were located in codon 12 (80.4%), G12D (39.1%), G12V (24.2%), G12S (6.5%), G12A (4.3%); G12C (4.3%), G12R (2.1%) and 19.6% at codon 13 (G13D). No differences were demonstrated in the frequency and distribution of mutations by gender, age, primary versus metastatic tumors or tumor location.Conclusions:
In this series, 42% of colorectal cancer tissue samples had KRAS mutations. Their frequency and distribution are similar to those reported in the literature, except for G12C mutation.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Rectal Neoplasms
/
Adenocarcinoma
/
Proto-Oncogene Proteins
/
Colonic Neoplasms
/
Ras Proteins
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Country/Region as subject:
South America
/
Chile
Language:
Spanish
Journal:
Rev. méd. Chile
Journal subject:
Medicine
Year:
2013
Type:
Article
Affiliation country:
Chile
Institution/Affiliation country:
Creative Bioscience/CL
Similar
MEDLINE
...
LILACS
LIS