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Endophytic fungi producing of esterases: evaluation in vitro of the enzymatic activity using pH indicator
Lisboa, Helen Cristina Fávero; Biasetto, Carolina Rabal; Medeiros, João Batista de; Araújo, Ângela Regina; Silva, Dulce Helena Siqueira; Teles, Helder Lopes; Trevisan, Henrique Celso.
  • Lisboa, Helen Cristina Fávero; Universidade Estadual Paulista Julio de Mesquita Filho. Instituto de Química. Araraquara. BR
  • Biasetto, Carolina Rabal; Universidade Estadual Paulista Julio de Mesquita Filho. Instituto de Química. Araraquara. BR
  • Medeiros, João Batista de; Universidade Estadual Paulista Julio de Mesquita Filho. Instituto de Química. Araraquara. BR
  • Araújo, Ângela Regina; Universidade Estadual Paulista Julio de Mesquita Filho. Instituto de Química. Araraquara. BR
  • Silva, Dulce Helena Siqueira; Universidade Estadual Paulista Julio de Mesquita Filho. Instituto de Química. Araraquara. BR
  • Teles, Helder Lopes; Universidade Estadual Paulista Julio de Mesquita Filho. Instituto de Química. Araraquara. BR
  • Trevisan, Henrique Celso; Universidade Estadual Paulista Julio de Mesquita Filho. Instituto de Química. Araraquara. BR
Braz. j. microbiol ; 44(3): 923-926, July-Sept. 2013. ilus, tab
Article in English | LILACS | ID: lil-699782
ABSTRACT
A sensitive and efficient colorimetric method was optimized for detection of esterase enzymes produced by endophytic fungi for development of High-Throughput Screening (HTS). The fungi were isolated and obtained previously from plant species of Cerrado and Atlantic Forest located in areas of environmental preservation in the State of Sao Paulo / Brazil, as part of the project "Chemical and biological prospecting endophytic fungi associated to plant species of Cerrado and Atlantic Forest". The compounds ethyl butyrate, ethyl acetate and methyl propionate were used as standards esters which were hydrolyzed by extracellular enzyme from endophytic fungi (EC. 3.1.1.1 -carboxylesterases) for production of carboxylic acids. Thus, the reduction of the pH increases the protonated indicator concentration (bromothymol blue), changing the color of the reaction medium (from blue to yellow), that can be observed and measured by spectrophotometry at 616 nm. The methodology with acid-base indicator was performed on 13 microorganisms, aiming Periconia atropurpurea asapotential source of esterase for biotransformation of short chain esters. The results also evidenced that this methodology showed to be efficient, fast, cheap, having low consumption of reagents and easy development, and can be applied to screen carboxylic-ester hydrolases in a large number of microorganisms.
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Full text: Available Index: LILACS (Americas) Main subject: Colorimetry / Esterases / Endophytes / Fungi Type of study: Evaluation studies Country/Region as subject: South America / Brazil Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2013 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual Paulista Julio de Mesquita Filho/BR

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Full text: Available Index: LILACS (Americas) Main subject: Colorimetry / Esterases / Endophytes / Fungi Type of study: Evaluation studies Country/Region as subject: South America / Brazil Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2013 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual Paulista Julio de Mesquita Filho/BR