Identification of Lactobacillus plantarum, Lactobacillus pentosus and Lactobacillus fermentum from honey stomach of honeybee
Braz. j. microbiol
;
44(3): 717-722, July-Sept. 2013. ilus, tab
Article
in English
| LILACS
| ID: lil-699804
ABSTRACT
This study aimed to isolate and identify Lactobacillus in the honey stomach of honeybee Apis dorsata. Samples of honeybee were collected from A. dorsata colonies in different bee trees and Lactobacillus bacteria isolated from honey stomachs. Ninety two isolates were Gram-stained and tested for catalase reaction. By using bacterial universal primers, the 16S rDNA gene from DNA of bacterial colonies amplified with polymerase chain reaction (PCR). Forty-nine bacterial 16S rDNA gene were sequenced and entrusted in GenBank. Phylogenetic analysis showed they were different phylotypes of Lactobacillus. Two of them were most closely relevant to the previously described species Lactobacillus plantarum. Other two phylotypes were identified to be closely related to Lactobacillus pentosus. However, only one phylotype was found to be distantly linked to the Lactobacillus fermentum. The outcomes of the present study indicated that L. plantarum, L. pentosus, and L. fermentum were the dominant lactobacilli in the honey stomach of honeybee A. dorsata collected during the dry season from Malaysia forest area - specifically "Melaleuca in Terengganu".
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Bees
/
Lactobacillus
Type of study:
Diagnostic study
Limits:
Animals
Country/Region as subject:
Asia
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2013
Type:
Article
Affiliation country:
Malaysia
Institution/Affiliation country:
University Putra Malaysia/MY
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