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Agrowaste-based Polyhydroxyalkanoate (PHA) production using hydrolytic potential of Bacillus thuringiensis IAM 12077
Gowda, Vaishnavi; Shivakumar, Srividya.
  • Gowda, Vaishnavi; Jain University. Center for PG Studies. Department of Microbiology. Jayanagar. IN
  • Shivakumar, Srividya; Jain University. Center for PG Studies. Department of Microbiology. Jayanagar. IN
Braz. arch. biol. technol ; 57(1): 55-61, Jan.-Feb. 2014. graf, tab
Article in English | LILACS | ID: lil-702570
ABSTRACT
The study identified the innate enzymatic potential (amylase) of the PHB producing strain B.thuringiensis IAM 12077 and explored the same for cost-effective production of PHB using agrowastes, eliminating the need for pretreatment (acid hydrolysis and/or commercial enzyme). Comparative polyhydroxyalkanoate (PHA) production by B. thuringiensis IAM 12077 in biphasic growth conditions using glucose and starch showed appreciable levels of growth (5.7 and 6.8 g/L) and PHA production (58.5 and 41.5%) with a PHA yield of 3.3 and 2.8 g/L, respectively. Nitrogen deficiency supported maximum PHA yield (2.46 g/L) and accumulation (53.3%). Maximum growth (3.6 g/L), PHB yield (2.6 g/L) and PHA accumulation (72.8%) was obtained with CN ratio of 81 using starch as the carbon source (10 g/L). Nine substrates (agro and food wastes) viz. rice husk, wheat bran, ragi husk, jowar husk, jackfruit seed powder, mango peel, potato peel, bagasse and straw were subjected to two treatments- acid hydrolysis and hydrolysis by innate enzymes, and the reducing sugars released thereby were utilized for polymer production. All the substrates tested supported comparable PHB production with acid hydrolysis (0.96 g/L-8.03 g/L) and enzyme hydrolysis (0.96 g/L -5.16 g/L). Mango peel yielded the highest PHB (4.03 g/L; 51.3%), followed by jackfruit seed powder (3.93 g/L; 29.32%). Varied levels of amylase activity (0.25U-10U) in all the substrates suggested the enzymatic hydrolysis of agrowastes.


Full text: Available Index: LILACS (Americas) Language: English Journal: Braz. arch. biol. technol Journal subject: Biology Year: 2014 Type: Article Affiliation country: India Institution/Affiliation country: Jain University/IN

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Full text: Available Index: LILACS (Americas) Language: English Journal: Braz. arch. biol. technol Journal subject: Biology Year: 2014 Type: Article Affiliation country: India Institution/Affiliation country: Jain University/IN