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Evaluation of the metabolism of glycosaminoglycans in patients with interstitial cystis
Lucon, Marcos; Martins, Joao Roberto; Leite, Katia Ramos Moreira; Soler, Roberto; Nader, Helena B.; Srougi, Miguel; Bruschini, Homero.
  • Lucon, Marcos; University of Sao Paulo Medical Schoo. Division of Urology. Sao Paulo. BR
  • Martins, Joao Roberto; University of Sao Paulo Medical Schoo. Division of Urology. Sao Paulo. BR
  • Leite, Katia Ramos Moreira; University of Sao Paulo Medical Schoo. Division of Urology. Sao Paulo. BR
  • Soler, Roberto; University of Sao Paulo Medical Schoo. Division of Urology. Sao Paulo. BR
  • Nader, Helena B.; University of Sao Paulo Medical Schoo. Division of Urology. Sao Paulo. BR
  • Srougi, Miguel; University of Sao Paulo Medical Schoo. Division of Urology. Sao Paulo. BR
  • Bruschini, Homero; University of Sao Paulo Medical Schoo. Division of Urology. Sao Paulo. BR
Int. braz. j. urol ; 40(1): 72-79, Jan-Feb/2014. tab, graf
Article in English | LILACS | ID: lil-704176
ABSTRACT

Introduction:

Painful bladder syndrome/interstitial cystitis (PBS/IC) pathogenesis is not fully known, but evidence shows that glycosaminoglycans (GAG) of bladder urothelium can participate in its genesis. The loss of these compounds facilitates the contact of urine compounds with deeper portions of bladder wall triggering an inflammatory process. We investigated GAG in urine and tissue of PBS/IC and pure stress urinary incontinence (SUI) patients to better understand its metabolism. Materials and

Methods:

Tissue and urine of 11 patients with PBS/IC according to NIDDK criteria were compared to 11 SUI patients. Tissue samples were analyzed by histological, immunohistochemistry and immunofluorescence methods. Statistical analysis were performed using t Student test and Anova, considering significant when p < 0.05.

Results:

PBS/IC patients had lower concentration of GAG in urine when compared to SUI (respectively 0.45 ± 0.11 x 0.62 ± 0.13 mg/mg creatinine, p < 0.05). However, there was no reduction of the content of GAG in the urothelium of both groups. Immunofluorescence showed that PBS/IC patients had a stronger staining of TGF-beta, decorin (a proteoglycan of chondroitin/dermatan sulfate), fibronectin and hyaluronic acid.

Conclusion:

the results suggest that GAG may be related to the ongoing process of inflammation and remodeling of the dysfunctional urothelium that is present in the PBS/IC. .
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Urinary Incontinence, Stress / Cystitis, Interstitial / Glycosaminoglycans Type of study: Evaluation studies Limits: Adult / Aged / Female / Humans Language: English Journal: Int. braz. j. urol Journal subject: Urology Year: 2014 Type: Article Affiliation country: Brazil Institution/Affiliation country: University of Sao Paulo Medical Schoo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Urinary Incontinence, Stress / Cystitis, Interstitial / Glycosaminoglycans Type of study: Evaluation studies Limits: Adult / Aged / Female / Humans Language: English Journal: Int. braz. j. urol Journal subject: Urology Year: 2014 Type: Article Affiliation country: Brazil Institution/Affiliation country: University of Sao Paulo Medical Schoo/BR