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Expresión y amplificación del gen HER2 en el cáncer gástrico avanzado / HER2 gene amplification and overexpression in advanced gastric cancer
Roa, Iván; Slater, Jeannie; Carvajal, Daniel; Schalper, Kurt; Toro, Gonzalo de; Ares, Raúl; Game, Anakaren; León, Jorge; Aretxabala, Xabier de.
  • Roa, Iván; Creative Bioscience. Santiago. CL
  • Slater, Jeannie; Creative Bioscience. Santiago. CL
  • Carvajal, Daniel; Creative Bioscience. Santiago. CL
  • Schalper, Kurt; Creative Bioscience. Santiago. CL
  • Toro, Gonzalo de; Creative Bioscience. Santiago. CL
  • Ares, Raúl; Creative Bioscience. Santiago. CL
  • Game, Anakaren; Creative Bioscience. Santiago. CL
  • León, Jorge; Creative Bioscience. Santiago. CL
  • Aretxabala, Xabier de; Creative Bioscience. Santiago. CL
Rev. méd. Chile ; 141(11): 1411-1419, nov. 2013. ilus, graf, tab
Article in Spanish | LILACS | ID: lil-704568
ABSTRACT

Background:

Overexpression/amplification of the HER2 gene in advanced gastric cancer is a predictor of response to adjuvant therapy with monoclonal antibodies.

Aim:

To determine the frequency of HER2 gene overexpression and amplificationin advanced gastric cancer. Material and

Methods:

One hundred nine advancedgastric cancer biopsy specimens, from 76 men and 33 women aged 67 ± 14 and 62± 12 years respectively, were selected. Three histological patterns (diffuse, intestinaland mixed) were recognized. Automated immunohistochemistry was performedwith monoclonal c-erbB-2 (NCL-356) Novocastra. Fluorescent in situ hybridization (FISH) for HER2 was performed in positive cases.

Results:

In 39% of cases,immunohistochemical staining was negative. It was 1+, 2+ and 3+ positive in 15,36 and 11% of cases, respectively. It was positive in 16% and 3% of intestinal typeand mixed carcinomas, respectively. It was negative in all diffuse carcinomas. FISHwas performed in 39 (2 +) cases and in 11 (3 +) cases. The gene amplification waspositive in two (2 +) and 11 (3 +) cases (11.9%). The overall concordance betweenimmunohistochemical staining and in situ hybridization was 85%.

Conclusions:

Inadvanced gastric cancer, HER2 gene overexpression or amplification was observed in11% and 12% of cases, respectively.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Stomach Neoplasms / Adenocarcinoma / Gene Amplification Limits: Aged / Female / Humans / Male Language: Spanish Journal: Rev. méd. Chile Journal subject: Medicine Year: 2013 Type: Article Affiliation country: Chile Institution/Affiliation country: Creative Bioscience/CL

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Full text: Available Index: LILACS (Americas) Main subject: Stomach Neoplasms / Adenocarcinoma / Gene Amplification Limits: Aged / Female / Humans / Male Language: Spanish Journal: Rev. méd. Chile Journal subject: Medicine Year: 2013 Type: Article Affiliation country: Chile Institution/Affiliation country: Creative Bioscience/CL