Extracellular methionine amino peptidase (MAP) production by Streptomyces gedanensis in solid-state fermentation
Braz. arch. biol. technol
;
57(2): 187-193, Mar.-Apr. 2014. graf, tab
Article
in English
| LILACS
| ID: lil-705744
ABSTRACT
A bioprocess was developed for extracellular MAP production from Streptomyces gedanensis by solid-state fermentation. Response surface methodology of Box Behken Design was performed to evaluate the interaction effects of most significant variables {inoculum size, (NH4)2SO4 concentration, MgSO4.7H2O and tryptone) on MAP production after the single parameter optimization and it resulted a maximum MAP production of 55.26 IU/g PUF after 120 h of fermentation. The concentrated crude MAP displayed a pH and temperature optimum of 8.5 and 50°C. By analyzing the thermal stability, the MAP was found to be stable in a temperature range of 50 to 55°C but lost about 50% of its activity at 65°C after 30 min. This is a first report of this kind of study for MAP.
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Index:
LILACS (Americas)
Language:
English
Journal:
Braz. arch. biol. technol
Journal subject:
Biology
Year:
2014
Type:
Article
Affiliation country:
India
Institution/Affiliation country:
National Institute for Interdisciplinary Science and Technology;/IN
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