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Fibrinogen and thrombin concentrations are critical for fibrin glue adherence in rat high-risk colon anastomoses
Buen, Eliseo Portilla-de; Orozco-Mosqueda, Abel; Leal-Cortés, Caridad; Vázquez-Camacho, Gonzalo; Fuentes-Orozco, Clotilde; Alvarez-Villaseñor, Andrea Socorro; Macías-Amezcua, Michel Dassaejv; González-Ojeda, Alejandro.
  • Buen, Eliseo Portilla-de; Surgical Research Division. Centro de Investigación Biomédica de Occidente, IMSS. Guadalajara. MX
  • Orozco-Mosqueda, Abel; Surgical Research Division. Centro de Investigación Biomédica de Occidente, IMSS. Guadalajara. MX
  • Leal-Cortés, Caridad; Surgical Research Division. Centro de Investigación Biomédica de Occidente, IMSS. Guadalajara. MX
  • Vázquez-Camacho, Gonzalo; Surgical Research Division. Centro de Investigación Biomédica de Occidente, IMSS. Guadalajara. MX
  • Fuentes-Orozco, Clotilde; Surgical Research Division. Centro de Investigación Biomédica de Occidente, IMSS. Guadalajara. MX
  • Alvarez-Villaseñor, Andrea Socorro; Surgical Research Division. Centro de Investigación Biomédica de Occidente, IMSS. Guadalajara. MX
  • Macías-Amezcua, Michel Dassaejv; Surgical Research Division. Centro de Investigación Biomédica de Occidente, IMSS. Guadalajara. MX
  • González-Ojeda, Alejandro; Surgical Research Division. Centro de Investigación Biomédica de Occidente, IMSS. Guadalajara. MX
Clinics ; 69(4): 259-264, 4/2014. tab, graf
Article in English | LILACS | ID: lil-705782
ABSTRACT

OBJECTIVE:

Fibrin glues have not been consistently successful in preventing the dehiscence of high-risk colonic anastomoses. Fibrinogen and thrombin concentrations in glues determine their ability to function as sealants, healers, and/or adhesives. The objective of the current study was to compare the effects of different concentrations of fibrinogen and thrombin on bursting pressure, leaks, dehiscence, and morphology of high-risk ischemic colonic anastomoses using fibrin glue in rats.

METHODS:

Colonic anastomoses in adult female Sprague-Dawley rats (weight, 250-350 g) treated with fibrin glue containing different concentrations of fibrinogen and thrombin were evaluated at post-operative day 5. The interventions were low-risk (normal) or high-risk (ischemic) end-to-end colonic anastomoses using polypropylene sutures and topical application of fibrinogen at high (120 mg/mL) or low (40 mg/mL) concentrations and thrombin at high (1000 IU/mL) or low (500 IU/mL) concentrations.

RESULTS:

Ischemia alone, anastomosis alone, or both together reduced the bursting pressure. Glues containing a low fibrinogen concentration improved this parameter in all cases. High thrombin in combination with low fibrinogen also improved adherence exclusively in low-risk anastomoses. No differences were detected with respect to macroscopic parameters, histopathology, or hydroxyproline content at 5 days post-anastomosis.

CONCLUSIONS:

Fibrin glue with a low fibrinogen content normalizes the bursting pressure of high-risk ischemic left-colon anastomoses in rats at day 5 after surgery. .
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Tissue Adhesives / Fibrinogen / Thrombin / Fibrin Tissue Adhesive / Colon / Ischemia Type of study: Etiology study Limits: Animals Language: English Journal: Clinics Journal subject: Medicine Year: 2014 Type: Article / Project document Affiliation country: Mexico Institution/Affiliation country: Surgical Research Division/MX

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Full text: Available Index: LILACS (Americas) Main subject: Tissue Adhesives / Fibrinogen / Thrombin / Fibrin Tissue Adhesive / Colon / Ischemia Type of study: Etiology study Limits: Animals Language: English Journal: Clinics Journal subject: Medicine Year: 2014 Type: Article / Project document Affiliation country: Mexico Institution/Affiliation country: Surgical Research Division/MX