Fibrinogen and thrombin concentrations are critical for fibrin glue adherence in rat high-risk colon anastomoses
Clinics
; 69(4): 259-264, 4/2014. tab, graf
Article
in En
| LILACS
| ID: lil-705782
Responsible library:
BR1.1
ABSTRACT
OBJECTIVE:
Fibrin glues have not been consistently successful in preventing the dehiscence of high-risk colonic anastomoses. Fibrinogen and thrombin concentrations in glues determine their ability to function as sealants, healers, and/or adhesives. The objective of the current study was to compare the effects of different concentrations of fibrinogen and thrombin on bursting pressure, leaks, dehiscence, and morphology of high-risk ischemic colonic anastomoses using fibrin glue in rats.METHODS:
Colonic anastomoses in adult female Sprague-Dawley rats (weight, 250-350 g) treated with fibrin glue containing different concentrations of fibrinogen and thrombin were evaluated at post-operative day 5. The interventions were low-risk (normal) or high-risk (ischemic) end-to-end colonic anastomoses using polypropylene sutures and topical application of fibrinogen at high (120 mg/mL) or low (40 mg/mL) concentrations and thrombin at high (1000 IU/mL) or low (500 IU/mL) concentrations.RESULTS:
Ischemia alone, anastomosis alone, or both together reduced the bursting pressure. Glues containing a low fibrinogen concentration improved this parameter in all cases. High thrombin in combination with low fibrinogen also improved adherence exclusively in low-risk anastomoses. No differences were detected with respect to macroscopic parameters, histopathology, or hydroxyproline content at 5 days post-anastomosis.CONCLUSIONS:
Fibrin glue with a low fibrinogen content normalizes the bursting pressure of high-risk ischemic left-colon anastomoses in rats at day 5 after surgery. .Key words
Full text:
1
Index:
LILACS
Main subject:
Tissue Adhesives
/
Fibrinogen
/
Thrombin
/
Fibrin Tissue Adhesive
/
Colon
/
Ischemia
Type of study:
Etiology_studies
Limits:
Animals
Language:
En
Journal:
Clinics
Journal subject:
MEDICINA
Year:
2014
Type:
Article
/
Project document