In Vitro antimicrobial photoinactivation with methylene blue in different microorganisms
Braz. j. oral sci
;
13(1): 53-57, Jan-Mar/2014. tab, graf
Article
in English
| LILACS
| ID: lil-709499
ABSTRACT
AIM:
To evaluate the in vitro antimicrobial effects of photodynamic therapy (PDT).METHODS:
The microorganism indicators were Candida albicans, Pseudomonas aeruginosa,Enterococcus faecalis and Staphylococcus aureus. A microbial pool was prepared (10 8 cells/mL), from which aliquots were transferred to culture plates for carrying out the PDT using methylene blue (50 µM) and low-power laser (660 nm, 100 mW and 9 J).The effect of methylene blue alone, low power laser and the absence of treatments were evaluated. Then, aliquots of 1 µL were plated in a media culture, the number of colony forming units (CFU/mL) was obtained and the data submitted to the F test (ANOVA) with Tamhane's comparisons.RESULTS:
The laser radiation in the presence of methylene blue was able to eliminate 74.90% of C. albicans, 72.41% of P. aeruginosa, 96.44% of E. faecalis and 95.42% of S. aureus, thus statistically significant differences were found among the groups (p<0.001).CONCLUSIONS:
PDT was effective in reducing the number of viable cells in the studiedmicroorganisms, especially E. faecalis and S. aureus.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Photochemotherapy
/
Pseudomonas aeruginosa
/
Staphylococcus aureus
/
Candida albicans
/
Enterococcus faecalis
/
Endodontics
/
Methylene Blue
/
Microbiology
Language:
English
Journal:
Braz. j. oral sci
Journal subject:
Dentistry
Year:
2014
Type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal de Pernambuco/BR
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