Synergistic antibacterial action of β-sitosterol-D-glucopyranoside isolated from Desmostachya bipinnata leaves with antibiotics against common human pathogens
Rev. bras. farmacogn
;
24(1): 44-50, Jan-Feb/2014. tab, graf
Article
in English
| LILACS
| ID: lil-710153
ABSTRACT
Desmostachya bipinnata (L.) Stapf, Poaceae, or Kusha in Sanskrit, is a sacred grass used extensively in Indian Vedic practices. It is well known for its medicinal value and is used in traditional Indian medicine to treat microbial infection in combination with other herbs. An effort has been made to isolate and characterize the bioactive compounds from the hydroalcoholic extract of D. bipinnata through bioassay guided fractionation, column chromatography. Their individual or combined antimicrobial properties were determined by the Resazurin Microtitre Assay, the checkerboard assay in combination with antibiotics, and by time kill curve analysis. β-Sitosterol-D-glucopyranoside was the bioactive compound identified to have the best antimicrobial activity (MIC 6-50 µg/ml) and it works synergistically with most antibiotics, especially with ciprofloxacin. Time kill curves showed that BS kills most of the pathogens within 5-10 h. To our knowledge at its best, this is the first time report of antibacterial synergy of β-sitosterol-D-glucopyranoside from D. bipinnata.
Full text:
Available
Index:
LILACS (Americas)
Language:
English
Journal:
Rev. bras. farmacogn
Journal subject:
Pharmacy
Year:
2014
Type:
Article
/
Project document
Affiliation country:
India
Institution/Affiliation country:
Sastra University/IN
Similar
MEDLINE
...
LILACS
LIS