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Mycobacteria mobility shift assay: a method for the rapid identification of Mycobacterium tuberculosis and nontuberculous mycobacteria
Wildner, Letícia Muraro; Bazzo, Maria Luiza; Liedke, Susie Coutinho; Nogueira, Christiane Lourenço; Segat, Gabriela; Senna, Simone Gonçalves; Schlindwein, Aline Daiane; Oliveira, Jaquelline Germano de; Rovaris, Darcita B; Bonjardim, Claudio A; Kroon, Erna G; Ferreira, Paulo CP.
  • Wildner, Letícia Muraro; Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular e Micobactérias. Florianópolis. BR
  • Bazzo, Maria Luiza; Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular e Micobactérias. Florianópolis. BR
  • Liedke, Susie Coutinho; Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular e Micobactérias. Florianópolis. BR
  • Nogueira, Christiane Lourenço; Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular e Micobactérias. Florianópolis. BR
  • Segat, Gabriela; Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular e Micobactérias. Florianópolis. BR
  • Senna, Simone Gonçalves; Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular e Micobactérias. Florianópolis. BR
  • Schlindwein, Aline Daiane; Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular e Micobactérias. Florianópolis. BR
  • Oliveira, Jaquelline Germano de; Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular e Micobactérias. Florianópolis. BR
  • Rovaris, Darcita B; Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular e Micobactérias. Florianópolis. BR
  • Bonjardim, Claudio A; Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular e Micobactérias. Florianópolis. BR
  • Kroon, Erna G; Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular e Micobactérias. Florianópolis. BR
  • Ferreira, Paulo CP; Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular e Micobactérias. Florianópolis. BR
Mem. Inst. Oswaldo Cruz ; 109(3): 356-361, 06/2014. tab, graf
Article in English | LILACS | ID: lil-711732
ABSTRACT
The identification of mycobacteria is essential because tuberculosis (TB) and mycobacteriosis are clinically indistinguishable and require different therapeutic regimens. The traditional phenotypic method is time consuming and may last up to 60 days. Indeed, rapid, affordable, specific and easy-to-perform identification methods are needed. We have previously described a polymerase chain reaction-based method called a mycobacteria mobility shift assay (MMSA) that was designed for Mycobacterium tuberculosis complex (MTC) and nontuberculous mycobacteria (NTM) species identification. The aim of this study was to assess the MMSA for the identification of MTC and NTM clinical isolates and to compare its performance with that of the PRA-hsp65 method. A total of 204 clinical isolates (102 NTM and 102 MTC) were identified by the MMSA and PRA-hsp65. For isolates for which these methods gave discordant results, definitive species identification was obtained by sequencing fragments of the 16S rRNA and hsp65 genes. Both methods correctly identified all MTC isolates. Among the NTM isolates, the MMSA alone assigned 94 (92.2%) to a complex or species, whereas the PRA-hsp65 method assigned 100% to a species. A 91.5% agreement was observed for the 94 NTM isolates identified by both methods. The MMSA provided correct identification for 96.8% of the NTM isolates compared with 94.7% for PRA-hsp65. The MMSA is a suitable auxiliary method for routine use for the rapid identification of mycobacteria.
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Full text: Available Index: LILACS (Americas) Main subject: Electrophoretic Mobility Shift Assay / Nontuberculous Mycobacteria / Mycobacterium tuberculosis Type of study: Diagnostic study Limits: Humans Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2014 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Santa Catarina/BR

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Full text: Available Index: LILACS (Americas) Main subject: Electrophoretic Mobility Shift Assay / Nontuberculous Mycobacteria / Mycobacterium tuberculosis Type of study: Diagnostic study Limits: Humans Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2014 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Santa Catarina/BR