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Chemopreventive effect of Brazilian green propolis on experimental dermal carcinogenesis in murine model / Efecto quimiopreventivo del propóleos verde brasileño sobre la carcinogénesis cutánea en el modelo murino experimental
Pereira-Filho, Rose Nely; Batista, Fellipe Santos; Ribeiro, Danielle Rodrigues; Melo, Genecy Calado de; Reis, Francisco Prado; Melo, Allan Ulisses Carvalho de; Gomes, Margarete Zanardo; Cardoso, Juliana Cordeiro; Albuquerque Júnior, Ricardo Luiz Cavalcanti de.
  • Pereira-Filho, Rose Nely; University Tiradentes. Science and Technology Institute. BR
  • Batista, Fellipe Santos; University Tiradentes. School of Dentistry. BR
  • Ribeiro, Danielle Rodrigues; University Tiradentes. Science and Technology Institute. BR
  • Melo, Genecy Calado de; University Tiradentes. School of Dentistry. BR
  • Reis, Francisco Prado; University Tiradentes. Science and Technology Institute. BR
  • Melo, Allan Ulisses Carvalho de; University Tiradentes. School of Dentistry. BR
  • Gomes, Margarete Zanardo; University Tiradentes. Science and Technology Institute. BR
  • Cardoso, Juliana Cordeiro; University Tiradentes. Science and Technology Institute. BR
  • Albuquerque Júnior, Ricardo Luiz Cavalcanti de; University Tiradentes. Science and Technology Institute. BR
Int. j. morphol ; 32(2): 522-530, jun. 2014. ilus
Article in English | LILACS | ID: lil-714303
ABSTRACT
The aim of this study was to assess the effect of oral administration of Hydroalcoholic Extract of Green Propolis (HEGP) on dermal carcinogenesis in rodent model. For the biological assay, we used 36 mice, assigned into 6 groups (n=6) CTR (treated with 100 mg/kg HEGP and no tumor induction), TUM (treated with water and tumor induction), GP10 (treated with 10 mg/kg HEGP and tumor induction), GP50 (treated with 50 mg/kg HEGP and tumor induction) and GP100 (treated with 100 mg/kg HEGP and tumor induction). Cancer induction was performed in the back of the mice by topical application of DMBA. After 16 weeks, mice were euthanized and their backs were submitted to post-mortem histological analysis. The mean number of lesions developed in TUM (4.14±0.89) was significantly higher than in GP10 (2.05±1.02), GP50 (1.8±1.92) and GP100 (2.5±1.73) (p<0.05). The tumors formed in HEGP-treated groups were histologically more differentiated, but only in PV100 in situ lesions were evidenced. Infiltration of anatomical noble structures was less frequent in HEGP-treated groups (p<0.05). Our data suggest that oral administration of HEGP provided partial inhibition of DMBA-induced dermal carcinogenesis, as well as appeared to modulate the differentiation and infiltrative potential of the carcinomas in rodent model.
RESUMEN
El objetivo de este estudio fue evaluar el efecto de la administración oral de extracto hidroalcohólico del propóleos verde (HEGP) sobre la carcinogénesis dérmica en modelo de roedores. Para el ensayo biológico, se utilizaron 36 ratones asignados en 6 grupos (n = 6) CTR (tratado con 100 mg/kg HEGP y sin inducción de tumores), TUM (tratada con agua e inducción de tumores), GP10 (tratado con 10 mg/kg HEGP e inducción de tumores), GP50 (tratado con 50 mg/kg HEGP e inducción de tumores) y GP100 (tratado con 100 mg/kg HEGP e inducción de tumores). La inducción de cáncer se llevó a cabo en la región dorsal de los ratones por aplicación tópica de DMBA. Después de 16 semanas, los ratones fueron sacrificados y sus dorsos fueron sometidos a análisis histológico post-mortem. El número medio de lesiones desarrolladas en TUM (4,14±0,89) fue significativamente mayor que GP10 (2,05±1,02), GP50 (1,8±1,92) y gp100 (2,5±1,73) (p<0,05). Los tumores formados en grupos tratados con HEGP fueron histológicamente más diferenciados, pero sólo en PV100 las lesiones in situ fueron manifiestas. La infiltración de las estructuras anatómicas blanco fue menos frecuente en los grupos tratados con HEGP (p<0,05). Nuestros datos sugieren que la administración oral de HEGP proporciona una inhibición parcial de la carcinogénesis dérmica inducida por DMBA, así como pareció modular la diferenciación y potencial infiltrante de los carcinomas en el modelo animal.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Propolis / Skin Neoplasms / Carcinogenesis Limits: Animals Country/Region as subject: South America / Brazil Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2014 Type: Article Affiliation country: Brazil Institution/Affiliation country: University Tiradentes/BR

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Full text: Available Index: LILACS (Americas) Main subject: Propolis / Skin Neoplasms / Carcinogenesis Limits: Animals Country/Region as subject: South America / Brazil Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2014 Type: Article Affiliation country: Brazil Institution/Affiliation country: University Tiradentes/BR