Búsqueda del ADN del virus HTLV-1 en biopsias de pacientes con linfoma cutáneo de células T / Detection of HTLV-1 DNA in biopsies of Chilean patients with cutaneous T-cell lymphoma
Rev. méd. Chile
;
142(3): 314-322, mar. 2014. ilus, tab
Article
in Spanish
| LILACS
| ID: lil-714355
ABSTRACT
Background:
Human T-lymphotropic virus-1 (HTLV-1) infection has been associated with the pathogenesis of cutaneous T cell lymphomas (CTCL).Aim:
To search for HTLV-1 DNA in skin biopsies of patients with CTCL. Material andMethods:
A retrospective study was conducted using 25 biopsies of patients with CTCL. DNA was extracted from lymphoid tissue by microdissection. A nested PCR was conducted to detect HTLV-1 genome using primers for the tax region. As negative controls, four cases of superficial perivascular dermatitis were chosen. As positive controls, five cases of T-cell leukemia/lymphoma (ATCL) were studied.Results:
A positive reaction was found in 3 of 25 cases. These biopsies corresponded to a case of Mycosis Fungoides, a case of CD30 (-) T-cell lymphoma and a case of lymphomatoid papulosis. Search was negative in the four cases of superficial perivascular dermatitis and positive in four cases of adult T-cell leukemia/lymphoma (ATCL).Conclusions:
HTLV-1 DNA search in tissues is a useful tool recommended to study T-cell lymphomas. HTLV-1 infection only occurs in sporadic cases but may contribute to tumor aggressiveness and prognosis.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Skin Neoplasms
/
DNA, Viral
/
Human T-lymphotropic virus 1
/
HTLV-I Infections
/
Lymphoma, T-Cell, Cutaneous
/
Mycosis Fungoides
Type of study:
Diagnostic study
/
Observational study
/
Risk factors
Limits:
Adult
/
Aged
/
Child, preschool
/
Female
/
Humans
/
Male
Country/Region as subject:
South America
/
Chile
Language:
Spanish
Journal:
Rev. méd. Chile
Journal subject:
Medicine
Year:
2014
Type:
Article
Affiliation country:
Chile
Institution/Affiliation country:
Pontificia Universidad Católica de Chile/CL
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