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Evaluation of Parkia pendula lectin mRNA differentially expressed in seedlings / Avaliação de mRNA da lectina de Parkia pendula diferencialmente expressa em plântulas
Rêgo, MJBM.; Santos, PB.; Carvalho-Junior, LB.; Stirling, J.; Beltrão, EIC..
  • Rêgo, MJBM.; Federal University of Pernambuco ? UFPE. Department of Pathology. Laboratory of Immunopathology Keizo Asami. Recife. BR
  • Santos, PB.; Federal University of Pernambuco ? UFPE. Department of Pathology. Laboratory of Immunopathology Keizo Asami. Recife. BR
  • Carvalho-Junior, LB.; Federal University of Pernambuco ? UFPE. Department of Pathology. Laboratory of Immunopathology Keizo Asami. Recife. BR
  • Stirling, J.; Federal University of Pernambuco ? UFPE. Department of Pathology. Laboratory of Immunopathology Keizo Asami. Recife. BR
  • Beltrão, EIC.; Federal University of Pernambuco ? UFPE. Department of Pathology. Laboratory of Immunopathology Keizo Asami. Recife. BR
Braz. j. biol ; 74(2): 489-492, 5/2014. tab, graf
Article in English | LILACS | ID: lil-719244
ABSTRACT
Parkia pendula (Willd.) Walp. (Fabaceae) is a neotropical species of the genus Parkia more abundantly distributed in Central to South America. From the seeds of P. pendula a glucose/mannose specific lectin (PpeL) was isolated that has been characterised and used as a biotechnological tool but until now this is the first manuscript to analyse P. pendula mRNA expression in seedlings. For this porpoise a Differential display reverse transcription polimerase chain reaction (DDRT-PCR) was used to evaluate the expression of P. pendula lectin mRNAs in non-rooted seedlings. No bands were observed in the agarose gel, indicating the absence of mRNA of PpeL seedlings. our findings confirm that lectins mRNAs are differently regulated among species even if they are grouped in the same class.
RESUMO
Parkia pendula (Willd.) Walp. (Fabaceae) é a espécie neotropical do gênero Parkia mais abundantemente distribuída na América Central a do Sul. Das sementes de P. pendula foi isolada uma lectina glicose/manose específica (Ppel) que foi caracterizada e usada como ferramenta biotecnológica, porém até o momento esse é o primeiro artigo a analisar a expressão do mRNA nas plântulas de P. pendula. Para esse propósito uma reação de PCR diferencial de transcriptase reversa (DDRT-PCR) foi utilizada para avaliar a expressão do mRNA da lectina de P. pendula em plântulas não enraizadas. Nenhuma banda foi observada no gel de agarose, indicando a ausência de mRNA das plântulas de PpeL. Nossos achados confirmam que os mRNAs de lectinas são regulados de forma diferentes entre as espécies, mesmo que sejam agrupadas na mesma classe.
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Full text: Available Index: LILACS (Americas) Main subject: RNA, Messenger / RNA, Plant / Plant Lectins / Fabaceae Language: English Journal: Braz. j. biol Journal subject: Biology Year: 2014 Type: Article Affiliation country: Brazil Institution/Affiliation country: Federal University of Pernambuco ? UFPE/BR

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Full text: Available Index: LILACS (Americas) Main subject: RNA, Messenger / RNA, Plant / Plant Lectins / Fabaceae Language: English Journal: Braz. j. biol Journal subject: Biology Year: 2014 Type: Article Affiliation country: Brazil Institution/Affiliation country: Federal University of Pernambuco ? UFPE/BR