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Sentinel lymph node biopsy in rats. Comparison between paraffin and frozen section analysis
Santos Filho, Ivan Dunshee de Abranches Oliveira; Enokihara, Milvia Maria Simões e Silva; Shie, Janaina Namba; Oliveira, Andrea Fernandes de; Santos, Ivan Dunshee de Abranches Oliveira; Ferreira, Lydia Masako.
  • Santos Filho, Ivan Dunshee de Abranches Oliveira; UNIFESP. Sao Paulo. BR
  • Enokihara, Milvia Maria Simões e Silva; UNIFESP. Sao Paulo. BR
  • Shie, Janaina Namba; UNIFESP. Sao Paulo. BR
  • Oliveira, Andrea Fernandes de; UNIFESP. Sao Paulo. BR
  • Santos, Ivan Dunshee de Abranches Oliveira; UNIFESP. Sao Paulo. BR
  • Ferreira, Lydia Masako; UNIFESP. Sao Paulo. BR
Acta cir. bras ; 29(supl.1): 57-61, 2014. tab
Article in English | LILACS | ID: lil-720396
ABSTRACT

PURPOSE:

To simulate a lymph node metastasis in an animal model using activated carbon, assess their identification in frozen section analysis and compare with histopathological examination in paraffin.

METHODS:

Thirty two adult female rats were used. They received the carbon injection on its hind legs. Half of the rats was sacrificed on day one, and the other half after 21 days. Thus, 64 lymph nodes were dissected and split longitudinally. One half of the lymph node was sent immediately to frozen section analysis. The other half was fixed in 10% formaldehyde to be cut in paraffin. Slides were divided into quadrants and classified by the presence of carbon in these four quadrants_ They were also classified by the carbon staining intensity.

RESULTS:

Comparing the slides obtained in the first day and 21 days, there was a tendency of carbon to spread over time, but without statistical significance. The intensity did not alter over time.

CONCLUSION:

There was no concordance between the two methods of pathological analysis, however the actived carbon was seen in all lymph nodes. .
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Paraffin Embedding / Sentinel Lymph Node Biopsy / Frozen Sections / Lymph Nodes Type of study: Prognostic study Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2014 Type: Article Affiliation country: Brazil Institution/Affiliation country: UNIFESP/BR

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Full text: Available Index: LILACS (Americas) Main subject: Paraffin Embedding / Sentinel Lymph Node Biopsy / Frozen Sections / Lymph Nodes Type of study: Prognostic study Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2014 Type: Article Affiliation country: Brazil Institution/Affiliation country: UNIFESP/BR